EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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DNA Extraction of a Musa acuminata Biology Lab Report \Analyze and Interpret A number of steps are required to isolate DNA from cellular contents. Describe what happens at each step‚ and why it acts to separate the parts of the cell. /6 There are three specific steps required to isolate DNA from its cellular contents. The steps used to remove and expose DNA from its cell are: breaking down the food type you are using by crushing it‚ for example a banana or strawberries‚ exposing the substance
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GENETICS (DNA - CHROMOSOMES) 7/16/2013 DNA – Chromosomes - Genes DNA •DNA: the chemical inside the nucleus of a cell that carries the genetic instructions for making living organisms. •The material inside the nucleus of cells that carries genetic information. • The scientific name for DNA is deoxyribonucleic acid. D.J.A 1 7/16/2013 http://www.ncbi.nlm.nih.gov/ Chromosomes • A chromosome is one of the threadlike "packages" of genes and other DNA in the nucleus of a cell. •
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DNA Extraction Lab Problem Statement: Do you think you have ever eaten DNA? Background Information: DNA is too small to see under a regular microscope‚ so how can it be studied? DNA is a large molecule found in all living things; therefore it is possible to extract it from cells or tissues. All we need to do is disrupt the cell’s plasma membrane and nuclear envelope‚ make the DNA clump together and - voila! - DNA extraction is possible. DNA extractions from onion‚ bananas‚ liver‚ or wheat
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RECOMBINANT DNA TECHNOLOGY * It is a technology that allows DNA to be produced via artificial means. * It is the joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science‚ medicine‚ agriculture‚ and industry. * Since the focus of all genetics is the gene‚ the fundamental goal of laboratory geneticists is to isolate‚ characterize‚ and manipulate genes. Although it is relatively
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: 1. Describe the structure of DNA. DNA stands for Deoxyribonucleic acid and looks like a spiral. The spiral is also known as a double helix. The strands are made up of our genetic information‚ composed of genes and chromosomes. There are four bases divided among purines and pyrimidines. On the purines there are Adenine (A) and Guanine (G). On the pyrimidines there are Cytosine (C) and Thymine (T). The
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Recombinant DNA Technology Benefits in Many Areas Recombinant DNA Technology is a DNA-based tool that allows scientists to find individual genes‚ cut them out‚ and insert them into the genome of another organism. Recombinant DNA Technology has been used to create different types of medicines for example human insulin. People with diabetes do not produce enough insulin for their own bodies‚ and in a lot of cases‚ they are allergic to non-human insulin. Due to the creation of Recombinant DNA Technology
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Strawberry DNA lab Extraction Purpose: The purpose of this lab experiment is to see if this particular type of fruit which in this case is a strawberry contains DNA. Using materials such as a Ziploc bag to help keep the entire strawberry in one place while it gets smashed and test tube to help better see the DNA and break down of the liquid in the left over strawberry sediments. Background information: The long thick fibers of DNA store the information for the function of the chemistry
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Deoxyribonucleic acid (DNA) is a molecule that encodes the genetic information used in the development and function of all known living organisms and some viruses. It is a major macromolecule that is essential for all known forms of life. Knowing this‚ several groups of scientists raced to find out how this essential molecule was structured. In an attempt to answer this question‚ James Watson and Francis Crick got together at the Cavendish Laboratory in Cambridge‚ England to experiment and record
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What role does DNA play in inheritance? - DNA is the genetic material of inheritance. Deoxyribonucleic acid (DNA) is the body’s instruction manual for making who you are. DNA is present in any living being. You receive one -half of your DNA from your money and one-half from you Father. People with light eyes tend to carry recessive alleles of the major gene and people with dark eyes tend to carry the dominant alleles. Genes are located on rodlike structures called chromosomes that are found in the
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