The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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TITLE: DNA Extraction from Banana DATE OF LABORATORY: 03/05/2013 LECTURER’S NAME: DR. LAM NYEE FAN DEMONSTRATORS’ NAME: MISS NOR EZANI AHMAD MISS LUSIA BAREK MOSES LABORATORY ASSISTANT NAME: MISS ROSILAH MOHD IDRUS STUDENT NAME AND MATRIC NUMBER: ELYAS ERIC HUIL(BS12110134) BONG SIN NENG(BS12110054) EDILAH NADRAH JOHANY( DIASSOFIA PAULA FRANKIE INTRODUCTION DNA is present in the cells of living oranisms. The nuclei acid DNA or deoxyribonucleic
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Watson and Crick discovering DNA Watson and Cricks discovery of DNA was important for many reasons because DNA made it possible to use genetic screening to identify which patients have ‘faulty’ genes which will lead to genetic illness such as Alzheimer’s. Watson and Crick discovered the structure of DNA in 1953. A reason that it was important was because it has provided an explanation for diseases and conditions that the germ theory cannot explain‚ for instance‚ cancer. This was important because
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The Role of DNA in Protein Synthesis DNA is found in the cells nucleus. It is part of the chromosome of the cell. DNA is the molecule that controls the making of protein. Protein is used for the growth of cells and organisms and also for the repair of cells. Protein is also used as an enzyme to speed up reactions in the body. Because of this DNA is able to control the whole organism. With out DNA and its ability to make protein the cells and eventually the whole organism would die
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Biotechnology Lab Report Lab: Extracting DNA from Bananas and Strawberries Purpose: To properly and successfully extract DNA from various fruits using cell disruption and separation techniques. Materials Used: 2 heavy duty zip-lock baggie 1 strawberry (fresh or frozen and thawed) 1 banana half 10 ml DNA extraction buffer* 2 Coffee filters Ice cold 95% ethanol 1 small beaker 2 Test tubes Wooden coffee stirrer *To make the extraction buffer‚ 100 ml of shampoo (without conditioner) was mixed
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store your DNA in a database? Not only that‚ how would you feel if you found out that your DNA was in the wrong hands? The boundary between violations of individual privacy can be quite difficult to identify due to a variety of reasons. DNA is the hereditary material that is the fundamental building block for an individual’s entire genetic makeup; it can be used in order to determine a wide range of information about an individual (Wall‚ 2005). DNA test banks are the storing of DNA information
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DNA replication is a biological process that occurs in all living organisms and copies their DNA; it is the basis for biological inheritance. The process starts when one double-stranded DNA molecule produces two identical copies of the molecule. The cell cycle (mitosis) also pertains to the DNA replication/reproduction process. The cell cycle includes interphase‚ prophase‚ metaphase‚ anaphase‚ and telophase. Each strand of the original double-stranded DNA molecule serves as template for the production
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are very helpful to you as you can see. Last but not least are nucleic acids. They consist of DNA‚ RNA‚ ATP etcetera. A difference between DNA and RNA is that DNA is double stranded and RNA is single stranded. The monomer of it is nucleotides. The structure is made up of sugar‚ phosphate group and a nitrogen Functions include the DNA containing all of your hereditary information and RNA uses your DNA to make proteins. This
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strands of DNA double helix are separated‚ each can serve as a template for the replication of a new complementary strand‚ producing two daughter molecules each of which contains two DNA strands with an antiparallel orientation. The enzymes involved in DNA replication process are template-directed polymerases that can synthesize the complementary sequence of each strand with extraordinary fidelity. This complex leads to the local denaturation and unwinding of an adjacent A + T rich region of DNA. The interaction
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Forensic DNA Profiling Forensic DNA Profiling Recent advancements in science and computer technology have allowed scientists and investigators to use genetics to aid in solving crime cases. Although there are many different types of methods used to analyze DNA‚ the general process is based upon the uniqueness of each individual’s DNA‚ much like a fingerprint. Due to this uniqueness‚ genetic evidence that matches a specific individual to a crime scene is often viewed as concrete and undeniable
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