General Biology I‚ Fall 2011 Lab Report 3 Name: Luan Nguyen Date: 11-30-11 Lab: Gel Electrophoresis Purpose of this lab: In this laboratory investigation‚ students will analyze hypothetical human DNA using restriction endonucleases and gel electrophoresis to match samples from a crime scene to a suspect. Introduction: Your analysis unit will use gel electrophoresis to determine the guilty party. Electrophoresis is a technology in science that allows an individual to separate
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GATTACA Viewing Questions – Answer Key 01. What deception is Vincent (main character) trying to hard to maintain? He is trying to be Jerome‚ who has an elite genetic identity. This identity gives Vincent the credentials to work at GATTACA. 02. Describe four ways that Vincent maintains his genetic identity. a. He uses Jerome’s blood‚ urine‚ and hair. b. He uses plastic surgery to change his face; cuts and lengthens his legs. c. He scrubs off all loose
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progeny by discrete‚ independent units‚ later called genes. His observations laid the groundwork for the field of genetics. 1869 Friedrich Miescher discovers a weak acid in the nuclei of white blood cells in the sperm of trout that today we call DNA. 1877 A technique for staining and identifying bacteria is developed by Koch. 1878 The first centrifuge is developed by Gustaf de Laval. 1879 In Michigan‚ Darwin devotee William James Beal makes the first clinically controlled crosses
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1. Outline the process of DNA profiling (genetic fingerprinting)‚ including ways in which it can be used. 6 marks • sample of DNA obtained / leucocytes / from mouthwash / hair / other named source • satellite DNA / repetitive sequences used for profiling • amplification of DNA by polymerase chain reaction / PCR • cutting DNA into fragments using restriction enzymes • separation of fragments of DNA (by electrophoresis) • separation according to the length of the fragments • pattern of
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out of molecules of DNA requires the use of special: A. digestive enzymes B. restriction enzymes C. enzymes from peroxisomes D. microscopic scalpels 2. To seal the cut fragments of DNA together‚ an enzyme called __________ is used. A. amylase B. peptidase C. trypsin D. ligase 3. Choose the correct sequence for gene transfer procedures. A. cleaving DNA‚ recombining DNA‚ cloning‚ screening
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Biomolecules - Organic compounds is first due to the fact that every word on the list is an organic compound‚ as they are essential to life processes. Next‚ carbohydrates‚ lipids‚ proteins‚ and nucleic acids are all macromolecules‚ as they are very large molecules composed of thousands of atoms or more. The map then divides into the four biomolecules. Carbohydrates are composed of monosaccharides‚ disaccharides‚ and polysaccharides. Proteins are made of amino acids‚ which are themselves made of polypeptides
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phenotypic outlook‚ and advances in biotechnology. This can be thought of as an evolutionary advantage to organisms in an unstable environment. Gene regulation in eukaryotes is a complicated process compared to prokaryotes. For example‚ 85% of the DNA is transcribed or translated in prokaryotes‚ while only 20% in eukaryotes. This reveals that transcription alone does not account for gene expression due to the complexity of eukaryotes.
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biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude‚ generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis‚ PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing‚ DNA-based phylogeny‚ or functional analysis of genes; the diagnosis of hereditary diseases; the
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1866 Mendel’s paper is published: units of inheritance in pairs; dominance and recessiveness; equal segregation; independent assortment. These ideas are not recognized for 34 years. 1869 DNA (first called "nuclein") is identified by Friedrich Miescher as an acidic substance found in cell nuclei. The significance of DNA is not appreciated for over 70 years. 1900 Mendel’s experiments from 1866 are "rediscovered" and confirmed by three separate researchers (one Dutch‚ one German‚ one Austrian). A British
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genomes and analyzing gene products. • The goal of sequencing genomes is to identify mutations in DNA and relate them to phenotypes (ie. Understanding genetics) • Human Genome Project- 13 year project‚ used chemically modified nucleotides • Next generation DNA sequencing- uses miniaturization techniques 1st developed for electronics industry‚ as well as principles of DNA replication and the polymerase chain reaction (PCR). • Massivley Parallel Sequencing- In next generation
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