BIO304: Forensic DNA Fingerprinting Lab DNA Fingerprinting‚ method of identification that compares fragments of DNA. It is sometimes called DNA typing. With the exception of identical twins‚ the complete DNA of each individual is unique. A DNA fingerprint is constructed by first extracting a DNA sample from body tissue or fluid such as hair‚ blood‚ or saliva. The sample is then segmented using enzymes‚ and the segments are arranged by size using a process called electrophoresis. The segments are
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Truman College Department of Biology Biology 121 Fall ‘14 Francisco Diaz de Leon Assistant Professor Office 2633 Phone: 773.907.4394 Email: fdiaz-deleon@ccc.edu Office Hours: Tuesday and Thursday 8:30 to 11:00 AM; Monday and Wednesday 11 AM to 12 PM; or by appointment. NOTICE: The best way to communicate with me outside the classroom is via email. I do not check phone messages regularly. Course Description: Cellular and molecular biology of the cell. Introduction to biochemistry
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Membrane transport and pumps; Cell cycle and cell growth control; Cell signaling and signal transduction; Biochemical and biophysical techniques for macromolecular analysis. Molecular Biology and Genetics: Molecular structure of genes and chromosomes; DNA replication and control; Transcription and its control; Translational processes; Regulatory controls in prokaryotes and eukaryotes; Mendelian inheritance; Gene interaction; Complementation; Linkage‚
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electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel matrix. In visualization‚ the gel is dyed so the results become present. This is used in modern crime labs‚ figuring out DNA‚ which plays a key role in many criminal trials. The researcher completed this experiment to figure out who committed the murder using gel electrophoresis. The researcher followed five steps in this experiment; First was placing the gel in the electrophoresis
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Which of the following is most likely to occur when a tumor-suppressor gene is mutated? - The tumor-suppressor gene and resulting protein may lose its function and ability to suppress cell proliferation. Mutations can produce a polypeptide with increased function. - TRUE ________can convert proto-oncogenes into oncogenes. - Nonsense mutations Most human embryos that are aneuploidy - are spontaneously aborted in the first trimester. Horses and donkeys are closely related species that can
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Biology – living organisms Scientific Method – making observations‚ proposing ideas about how something works‚ testing ideas‚ discarding or modifying -allows us to solve problems and answer questions efficiently and effectively. -Ideas about “how things work” = hypothesis. -proposed explanation for one or more observations -scientific hypothesis MUST be testable -must be falsifiable (observations could prove false) Observations Question Hypothesis = imagination‚ intuition‚ chance
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GENETICS JARGON Alleles (allelomorphs) Alternative forms of a particular gene. There can be several alleles of a particular gene in the population‚ but only 2 alleles can be inherited by a single individual. For simplicity much literature aimed at the general reader uses "gene" where it means "allele". Allelic Series A series of alleles that are all mutant forms of the same gene and are at the same locus (same position on the chromsome). Alternate Heredity Mendelian inheritance involving
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lives. The HGP involved the discovery of the full Deoxyribonucleic Acid (DNA) complement in a single human somatic cell. The project purpose was to sequence this sequence of DNA so that a map of it may be produced that shows the location and arrangement of all of the base pairs present in a human being. The HGP initial goals were to: * identify all the approximately 20‚000-25‚000 genes in human DNA * determine the sequences of the 3 billion
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What is Cloning? Clones are organisms that are exact genetic copies. Every single bit of their DNA is identical. Clones can happen naturally—identical twins are just one of many examples. Or they can be made in the lab. Below‚ find out how natural identical twins are similar to and different from clones made through modern cloning technologies. Many people first heard of cloning when Dolly the Sheep showed up on the scene in 1997. Artificial cloning technologies have been around for much longer
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Ballistic Fingerprinting Technology Michael GS1145 Unit 2 Assignment 2 Introduction In light of sniper shootings being on the rise for instance in the Washington area‚ four states are taking serious consideration in a new technology of creating a ballistic fingerprinting system enabling law enforcement the ability to trace shell casings found at crime scenes back to the maker and or buyer of the fire arm
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