Fritsch EF‚ Maniatis T. Molecular Cloning: a Laboratory Manual‚ 2nd ed.‚ Cold Spring Harbor Laboratory‚ Cold Spring Harbor‚ N.Y.‚ 1989. Basic Tools for DNA Manipulation and Molecular Genetic Analysis Enzymes Vectors Gel Electrophoresis PCR Southern blotting Gel Electrophoresis Gel is a complex net work of polymeric molecules Both DNA and RNA are negatively charged owing to phosphate backbone Nucleic acids migrate in a gel toward anode (+) at a rate that is inversely proportional to the log10
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1a: Nucleic acids Our class on DNA is divided into 3 parts: (I) Genetics (II) DNA structure (III) Concepts and applications. I. Genetics: In the primordial period‚ simple molecules were formed from atoms and from these molecules‚ macromolecules were formed. These macromolecules formed life and all living organisms. The classical genetic and heredity observations in the 19th century started the search for the origin of life. The transforming principle of DNA was demonstrated from the experiment
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at the DNA sequence level. The principle behind the technology rests on the possibility of comparing band profiles generated after restriction enzyme digestion in DNA molecules of different individuals. Diverse mutations that might have occurred affect DNA molecules in different ways‚ producing fragments of variable lengths. These differences in fragment lengths can be seen after gel electrophoresis‚ hybridisation and visualisation. Isolating DNA Isolating DNA is the first step for many DNA-based
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Wheat Germ DNA Isolation Darius Xavier Johnson 4/4/2014 Purpose: The purpose of this experiment was to isolate the DNA of wheat germ cells and to analyze that DNA for absorption rates when denatured and annealed. Reagent Table: Reagent Molecular Weight (g/mole) Boiling Point (°C) MeltingPoint (°C) Density Hazards NaCl 58.44 1413 801 2.165 g/ml Skin and eye irritant Inhalation Ethanol 46.07 78.5 -114.1 1.59 g/ml Skin and eye irritant Introduction: Chromatin
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heat-killed S cells with living R cells. | Avery‚ McCarty‚ and MacLeod | First to show that DNA was the genetic material‚ but not believed. Repeated Griffith’s experiment‚ but subjected the strains to different enzymes‚ isolating RNA‚ DNA‚ lipids‚ carbohydrates‚ and proteins. Only the DNA killed the mice; the others had the mice survive. | Hershey and Chase | Used T4 bacteriophages in radioactive phosphorus to track DNA and radioactive sulfur to track proteins to determine what is the transformational material
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Alana Corona period: Question Can you extract a DNA from a strawberry‚ if so will you be able to see the DNA without using a microscope? Purpose My purpose is to see if the DNA from a strawberry can be extracted by using house hold chemicals. Research All living things come with instructions stored in their DNA‚ short for deoxyribonucleic acid. Whether you are a human‚ rat‚ tomato‚ or bacteria‚ each cell will have DNA inside of it. DNA is the blueprint for everything that happens inside
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In chapter 11‚ we learned about DNA and Genes (11.1DNA: The Molecule of Heredity; 11.2From DNA to Protein; 11.3Genetic changes). DNA (deoxyribonucleic acid)‚ which is in a shape called a double helix‚ developed its name from the simple sugar in DNA (deoxyribose). The phosphate group is composed of one atom of phosphorus surrounded by four oxygen atoms. A nitrogen base is a carbon ring structure that contains one or more atoms of nitrogen. Next to cover is DNA replication in which enzyme breaks
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the polymer is DNA. If the sugar is ribose‚ the polymer is RNA. Together with proteins‚ nucleic acids are the most important biological macromolecules The two nucleic acids used in repair‚ reproduction and protein synthesis are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). RNA maybe found throughout the cell II.CLASSES DEOXYRIBONUCLEIC ACID (DNA) nucleic acid containing the genetic instructions used in the development and functioning of all known living organisms DNA is usually found
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Name: ______________________________________ Date: ________________________ Student Exploration: DNA Fingerprint Analysis Vocabulary: codon‚ DNA‚ DNA fingerprint‚ genotype‚ identical twins‚ nitrogenous base‚ phenotype‚ trait Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. The two navy officers shown at left are identical twins. Why do you think identical twins look so similar? _______________________________________________
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CRISPR is a new technology‚ a method that can edit genomes. It makes it possible for scientists to manipulate and make changes to the DNA in the cells of all organisms. Since this is such a new discovery‚ there hasn’t been done much testing and experimenting‚ which makes it unknown and none predictable for humans. It is said that with CRISPR‚ scientists will for example‚ be able to change genes in human embryos‚ and create so called “designer-babies”. There has been a lot of discussion around this
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