Conclusions of DH5α DNA transformation with red colonies resistance to ampicillin and the lacZ gene Introduction: In this experiment‚ a plasmid with a gene that has resistance to the antibiotic ampicillin and has lacZ is used to transfer the resistance into E. coli bacteria in red colonies. This same technique is used to give diabetics their insulin‚ and to give dwarfs growth hormones. The point of this lab is to give the groups an idea how DNA can be transformed
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Transformation of Bacterial Cells with Plasmid DNA Introduction: Transformation refers to the process in which the cell integrates foreign DNA to its genetic code‚ meaning it takes the genes and incorporates them into the cell’s current DNA. Cells that can do this naturally‚ most commonly bacteria and archea‚ are known as competent. The bacteria E. coli do not have high transformation competence under normal conditions‚ but can be manipulated to produce better results using
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Year 2 Date: 7th- 14th of February 2014. Title: Ligation of Lambda DNA pre-digested with EcoRI and HinDIII. Restriction of Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins
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The Effect of Varying Ligation Conditions on Ligation Efficiency Introduction The goal of this experiment was to successfully ligate RFP into P1 and transform E. coli with P1-RFP plasmid‚ which contains the P1 promoter‚ RBS‚ and RFP gene. We tested several different ligation conditions and assessed how they each affected ligation efficiency. The two variables that we tested were: molar ratio of insert (RFP): vector (P1 plasmid)‚ as well as the effect of removing the stuffer from the digested plasmid
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Ligation is the process of joining two DNA fragments or other molecules by a phosphate ester linkage with the action of enzyme. Ligation of DNA is process that involve the DNA of interest is inserted into the plasmid. This 3’-hydroxyl of DNA terminus are joined together with 5’-phosphoryl of another by phosphodiester bond. The ligation of DNA fragments usually performed by using T4 DNA ligase. All the reaction components such as ligation buffer‚ DNA insert‚ pGEM®-T‚ and T4 DNA ligase is mixed by
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Post Tubal Ligation Syndrome Are there truly side effects caused by tubal ligation? Candy Layne COM/220‚ Research Writing Instructor Amy McKenzie July 15‚ 2009 Post Tubal Ligation Syndrome Are there truly side effects caused by a tubal ligation? Ever heard of side effects from a simple surgery? How about the procedure causing harsh menstrual cycles? Thousands of women across the U.S. have had this surgery and have the same complaint. The surgery is a tubal
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If you wanted to have a baby and you had a tubal ligation‚ then what would be our choices? Most probably that would be IVF or tubal reversal. There will be number of choices that you can make. Like first if your doctor doesn’t know about tubal Reversal and go through IVF‚ 2nd is if your doctor know about Tubal Reversal but still insists of IVF‚ why a doctor will do that there are reasons if your doctor doesn’t know about Tubal Reversal the reason is that he didn’t take training in it. There is no
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DNA Cloning PCB3063L Section DNA cloning refers to the process of making multiple copies of a DNA fragment. For the past weeks we have conducted a set of experiments that allow us to clone a specific gene in drosophila. First we started by the process of DNA extraction‚ which allowed us to isolate the genomic DNA from D. Melanogaster. This process requires the use of lysis in other to extract the DNA and RNA. After extracting the DNA‚ we it is important to use
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Long stands of double helical DNA can fit into the nucleus of a single cell because DNA is specially packaged through a series of compaction events to fit easily within cell nuclei. Even though the length of DNA per cell is about 100‚000 times as long as the cell itself‚ it only takes up only about 10 percent of the cell’s volume. The DNA molecule‚ in order to condense‚ wraps itself around groups of histone proteins‚ and then the chromatin folds back on it‚ nucelosomes pack together to create a compact
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Recombinant DNA Report Our final annotated gel image sums up the successful experiments we performed over the course of 8 weeks. The image will be referred to throughout the report: Lane 1: 10 µL of ladder. Lane 2: 20 µL of a pAMP- EcoRI/HindIII double digestion. Within the double digestion‚ one can find 8 µL of pAMP‚ 1 µL of the EcoRI enzyme‚ 1 µL of the HindIII enzyme‚ 5 µL of 10x Buffer 2.1‚ and 35 µL of water. A total volume of 50 µL was present
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