Chapter 9 DNA-Based Information Technologies Multiple Choice Questions 1. DNA cloning: the basics Page: 307 Difficulty: 1 Ans: C Restriction enzymes: A) act at the membrane to restrict the passage of certain molecules into the cell. B) are highly specialized ribonucleases that degrade mRNA soon after its synthesis. C) are sequence-specific DNA endonucleases. D) are very specific proteases that cleave peptides at only certain sequences. E) catalyze the
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We wanted to extract‚ see and analyze DNA from a single strawberry( 12.11 g). The long‚ thick fibers of DNA store the information for the functioning of the chemistry of life. DNA is present in every cell of plants and animals. The DNA found in strawberry cells can be extracted using common‚ everyday materials. To start the procedure we needed the following supplies; one strawberry‚ one plastic cup‚ 10 ml of Cell Lysis Buffer (10% detergent‚ 1% NaCl)‚ one plastic zip-lock bag‚ one coffee filter‚
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In this coursework I will be exploring two issues‚ my major issue being DNA Fingerprinting and my minor issue is PCR (Polymerase Chain Reaction). DNA Fingerprinting (Obtained from www.anselm.edu/.../genbio/geneticsnot.html) (The diagram above shows that the defendant had the victim’s blood on his clothes) Web Description: A method of comparing the genetic similarities or differences between individuals. This technology is often used as a forensic tool to identify the source of blood
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March 3‚ 2013 Wrongful convictions. | How the use of DNA can exonerate those wrongfully convicted. Imagine wasting years of your life in a jail cell on death row‚ for a crime you did not commit. You have to ask yourself “how could this happen? How did an innocent person get convicted if indeed they are innocent?” Those are just a few questions you think of when you think of wrongful convictions. Some questions can be answered by the common causes of wrongful convictions‚ such as‚ eyewitness
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The ability of humans to manipulate DNA is a mixed blessing Genetically modified organisms are made moving the DNA from one species to another. DNA contains genes which code for proteins. For plants and bacteria; restriction enzymes are used to cut a DNA segment from a desired gene source and a bacterium plasmid. They are joined using ligase enzymes and inserted into the plant chromosome or host bacterium. For animals the recombinant DNA is injected into an embryo and transferred to a recipient
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of genomic DNA from animal tissues Introduction A rapid expansion of DNA analysis in medical‚ biotechnological and basic research has created the need for simple and efficient commercial methods for isolating genomic DNA. Many different methods and technologies are available for the isolation of genomic DNA. In general‚ all methods involve disruption and lysis of the starting material followed by the removal of proteins and other contaminants and finally recovery of the DNA. Removal of
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3.2.4 Genomic DNA extraction of the root tips After the treatment of the onion bulbs with lake water sample at different period of time‚ the DNA from the root tips will be ready to be extracted and observed. First of all‚ the genomic of the Allium cepa will be easily done by the already available Uneasy® Plant Mini kit (Qiagen‚ Germany) in the laboratory. All of the procedures can be performed by referring to the manual provided by the kit. A very small amount of root tips that weigh 100 mg will
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on on studying DNA by using a technique called X-ray diffraction. Her expertise was the X-ray diffraction mostly of proteins and lipids in a solution on DNA fibers. Franklin and a student‚ Raymond Gosling‚ took pictures of DNA and made the observation that there are two forms of DNA; the “A” form‚ which is dry and the”B” form‚ which is wet. One of their pictures of this observations‚ known as Photograph 51‚ became famous and was analytical evidence in discovering the structure of DNA. the photograph
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T DNA IN CRIMINAL INVESTIGATION 5Transportation and storage of DNA evidence is also extremely important. Whentransporting DNA evidence the officer should be aware that having the evidence in directsunlight can cause the evidence to become compromised (DNA Evidence‚ 2012). The officershould ensure that they do not place the evidence in an environment where it can get hot‚ insteadthey should place it in a cold environment to preserve it (DNA Evidence‚ 2012). It is importantthat the DNA evidence be
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Destinie Bradford Bio 1040 18 February 2013 Critical Analysis of DNA Replication in Bacteria DNA replication is a biological process that occurs in all living organisms and copies their DNA; it is the basis for natural inheritance. The process starts when one double-stranded DNA molecule produces two identical copies of the molecule. The cell cycle (mitosis) also pertains to the DNA replication/reproduction process. DNA replication‚ in eukaryotes‚ is controlled within the context of the cell cycle
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