Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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TERTIARY EDUCATION EQUIVALENCY & ACCREDTATION PROGRAM (ETEEAP) COLLEGE OF ENGINEERING ENVIRONMENTAL ENGINEERING CEAB3312 ASSIGNMENT NO# 1 ENVIRONMENTAL DEGRADATION‚ BIOCHEMICAL CYCLE AND ECOSYSTEM CYCLE MR. DANILO L. AUREADA Internal Assessor Instructor XIV SUBMITTED BY: MR. GABBY M. SANTAMARIA ETEEAP-BSIE Biochemical Cycles Table of Contents 1. Eutrophication 2. Contributors Plants such as trees and algae undergo the photosynthesis reaction where carbon dioxide and water in the
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1. PCR . 2. Protein extraction and purification . 3. Protein concentration determination . 4. SDS-PAGE . 1. The aim of experiments : 2.1 The aim of PCR experiment is to replicate some DNA dimmers by using specific enzymes used for replication in vitro which is done in lab not by living organisms. 2.2 The aim of protein extraction and purification experiment is to extract some proteins and purify them by specific methods. 2.3 The aim of concentration
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Associate Program Material DNA Worksheet Answer the following in at least 100 words: Describe the structure of DNA.DNA is thread formed by two strands‚ related together to form a double helix. The double helix looks like a twisted ladder. The sides of this ladder are long unites called nucleotides and are made of three parts; a nitrogenous base‚ a sugar‚ and a phosphate group. The sides of the ladder or the nucleotides from the two separate strands of the DNA are attached by an appendage
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electrophoresis machine. An electric current is run through the machine and the different sized molecules form bands on the gel matrix. In visualization‚ the gel is dyed so the results become present. This is used in modern crime labs‚ figuring out DNA‚ which plays a key role in many criminal trials. The researcher completed this experiment to figure out who committed the murder using gel electrophoresis. The researcher followed five steps in this experiment; First was placing the gel in the electrophoresis
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Extraction of DNA Molecule of an Onion In an Aqueous Solution By: Introduction DNA(deoxyribonucleic acid) is found in every living organism. It provides the information and instructions to build and regulate cells in organisms. The information it contains is used when organisms are being reproduced. It is made up of two polynucleotide chains known as DNA strands. Within the two chains there are four nucleotide units: adenine‚ thymine‚ cytosine‚ and guanine. Materials and Methods An onion
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TITLE : IDENTIFICATION OF BIOCHEMICALS IN PURE FORM AND IN COMMON FOODSTUFFS AIM: THE OBJECTIVE OF THIS EXPIREMENT IS TO PERFORM TESTS ON A VARIETY OF COMMON FOODS TO IDENTIFY THE CHEMICALS THEY CONTAIN AND TO TEST PURE SAMPLES OF BIOCHEMICALS TO GAIN FAMILIARITY WITH THE COLOUR CHANGES. RESULTS: TABLE 1 : BIOCHEMICAL TESTS ON PURIFIED BIOLOGICAL SUBSTANCES SAMPLE | TEST | OBSERVATION | Glucose | Reducing Sugar | The initial blue colouration of the mixture turns green
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DNA Notes Phil‚ Leah‚ John Tate‚ Danny‚ Cathy‚ Lou‚ Mark‚ Jan‚ Richard‚ Brian * The very beginning (page 9)‚ Jan is shocked when Mark says ‘dead’. * Page 10‚ introduction of Phil; first thing we learn about him is him eating ice cream whilst sitting next to Leah in a field. * Leah wonders and asks what is running through Phil’s mind – suggests she is concerned for him. * Phil does not reply; shows either he enjoys having her give him attention and wants her to keep on wondering
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Carbohydrates have two major functions in plants and animals: they serve as fuel and building material. Plants store most of their energy in the form of starch. Starch is a polysaccharide (consisting of several conjoined sugars). Synthesizing starch enables the plant to stockpile surplus glucose‚ and since glucose is a major cellular fuel‚ starch represents stored energy. The sugar can later be withdrawn by hydrolysis‚ which breaks the bonds between monomers. Animals‚ in turn‚ have enzymes that
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Tools and techniques for DNA manipulation Gene technology is the artificial manipulation of genes Gene technology’s use different techniques: TOOLS: Restriction enzyme Enzyme that are used to cut DNA at specific sequences ‘Like a pair of scissors’ One of the most important tools in genetic engineering They have the ability to cut DNA molecules at precise sequences of 4 to 8 base pairs called recognition sites A lot of bacteria are used to get restriction enzymes Bacteria use restriction
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