The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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Using DNA in Science and Technology DNA is the blueprint for life‚ it is found in the nucleus of cells on the chromosomes. It is found in many cell organelles‚ such as plasmids in bacteria‚ chloroplasts in plants‚ and mitochondria in both plants and animals. DNA helps us to perform many actions in solving crimes‚ and also helps us to do scientific studies that were not possible until recently. DNA has a double helix structure‚ its sugar phosphate backbone‚ made up of deoxyribose and a phosphate
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DNA- is the material found in the nucleus that contains the genetic info. 4 billion codes in length Gene- a segment of DNA that controls a certain protein production. A gene is made up of hundreds to thousands of codes. Chromosomes- genetic material that is coiled up into structures during cell division. Importance of DNA- genes control the production of proteins..(look like‚ body function‚ body communications‚ and enzyme control) DNA screening- the process of testing individuals to determine
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Experiment 2 Titles Extraction of Bacterial Plasmid DNA and Analysis of Extracted DNA Samples Objectives * To learn the procedures needed in extracting the bacterial plasmid DNA * To determine the concentration of original DNA sample and purity of prepared DNA sample by using spectrophotometer * To analyze the extracted DNA sample by gel electrophoresis Materials and methods (Refer to UDBB2144 Laboratory 2A Manual Principles of biotechnology page 6-10) Results
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their 1953 Nature publication‚ Watson and Crick announced their landmark discovery: DNA exists in the form of a right handed‚ three-dimensional double helix. They described their DNA model as two DNA strands connected by hydrogen bonds between complementary bases. Adenine bases are always paired with thymines‚ and cytosines are always paired with guanines. Watson and Crick identified the anti-parallel configuration of DNA strands; each 5’ end of one strand is paired with the 3’ end of its complementary
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Identification of Unknown Carbohydrates using various biochemical tests Date of experiment: 11/10/2012 Name of Student: Vicki Marshall Introduction A series of biochemical tests was carried out to identify unknown carbohydrates. There were six carbohydrates that needed to be identified; they have been randomly labelled A-F. The carbohydrates are glucose‚ fructose‚ maltose‚ lactose‚ sucrose and starch. There was six tests that were carried out to help identify them‚ these were: Iodine Test
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External links Photo guide: Round the clock at Dainik Bhaskar (http://www.printweek.com/india/news/1009865/PhotoGuide-Round-clock-Dainik-Bhaskar/) Dainik Bhaskar(Hindi) (http://www.bhaskar.com/) Divya Bhaskar(Gujarati) (http://www.divyabhaskar.co.in/) DNA(English) (http://www.dnaindia.com/) Prospectus (http://www.sebi.gov.in/dp/dbfinal.pdf) Retrieved from "http://en.wikipedia.org/w/index.php?title=Dainik_Bhaskar&oldid=567749943" Categories: Hindi-language newspapers Media in Bhopal Media of Rajasthan
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Gene Cloning Methodology of DNA What is DNA? DNA was discovered by the Swiss biochemist‚ Johann Friedrich Miescher‚ in 1869‚ while he was working in Tubingen‚ Germany. He found that the DNA molecule is large; acidic in nature and rich in phosphorus‚ but only in the 1930s was the real and complex structure of DNA fully studied. DNA (deoxyribonucleic acid) is the genetic material in all prokaryotes and eukaryotes‚ i.e. it is the material responsible for the transfer of hereditary traits from
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TITLE: DNA Extraction from Banana DATE OF LABORATORY: 03/05/2013 LECTURER’S NAME: DR. LAM NYEE FAN DEMONSTRATORS’ NAME: MISS NOR EZANI AHMAD MISS LUSIA BAREK MOSES LABORATORY ASSISTANT NAME: MISS ROSILAH MOHD IDRUS STUDENT NAME AND MATRIC NUMBER: ELYAS ERIC HUIL(BS12110134) BONG SIN NENG(BS12110054) EDILAH NADRAH JOHANY( DIASSOFIA PAULA FRANKIE INTRODUCTION DNA is present in the cells of living oranisms. The nuclei acid DNA or deoxyribonucleic
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Eddie Lai Clark 7 12/15/11 Cell Division/ DNA / Protein Synthesis Study guide AA: Simple definition AA: Simple explanation AA: Detailed explanation/drawing AA: Questions 1. What is transformation? * Movement of a gene from one organism to another 2. What did Griffith show? * Showed either protein or DNA causes transformation 3. What did Avery show? * Showed that DNA causes transformation or that DNA is hereditary material 4. What did Hershey & Chase show
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