DNA helicase -separates strands of nuclei acid‚ breaks H bond between nitrogenous bases.‚ works at the replication fork -DNA PRIMASE- lays RNA primer ‚ acts as new strand‚ can only add nucleotides to a free3’ end ‚ lays nucleotide with a 5’ orientation -DNA POLYMERASE 3- adds nucleotides using base pair rules lcreating 2 new daughter strands‚ only adds to a free 3’end and lays down nucleotide with 5’ orientation. Pol3 continuously synthesizes new daughter cell(leading strand) same direction as
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strands of DNA double helix are separated‚ each can serve as a template for the replication of a new complementary strand‚ producing two daughter molecules each of which contains two DNA strands with an antiparallel orientation. The enzymes involved in DNA replication process are template-directed polymerases that can synthesize the complementary sequence of each strand with extraordinary fidelity. This complex leads to the local denaturation and unwinding of an adjacent A + T rich region of DNA. The interaction
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1. PCR . 2. Protein extraction and purification . 3. Protein concentration determination . 4. SDS-PAGE . 1. The aim of experiments : 2.1 The aim of PCR experiment is to replicate some DNA dimmers by using specific enzymes used for replication in vitro which is done in lab not by living organisms. 2.2 The aim of protein extraction and purification experiment is to extract some proteins and purify them by specific methods. 2.3 The aim of concentration
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DNA Replication DNA replication is a biological process that occurs in all living organisms and copies their DNA. The initiation of DNA replication starts with two steps. First an initiator protein unwinds a short stretch of the DNA double helix. Then a protein called helicase attaches to and breaks apart the hydrogen bonds between the bases on the DNA strands‚ pulling apart the two strands. DNA replication starts when one double-stranded DNA molecule produces two identical copies of the molecule
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Lambda DNA Amplification by Polymerase Chain Reaction (PCR) Introduction/ Background* Since its introduction in 1985‚ polymerase chain reaction (PCR) has become a powerful tool in molecular genetic analysis. Today‚ it is used for applications such as cloning‚ analysis of DNA from ancient specimens‚ and analysis of human DNA for forensic applications. PCR is a test-tube DNA replication system for making many‚ many copies of‚ or amplifying‚ a defined segment of DNA. Using PCR‚ a selected target
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DNA bender: Cren7 & Sul7 DNA benders can introduce a bend in the DNA (Luijsterburg et al.‚ 2008). Many bends in the DNA automatically provide compaction of the DNA. Two important DNA bending proteins in crenarchaea are Cren7 and Sul7 (Driessen et al.‚ 2013). They are similar in structure‚ but they have different DNA binding regions (Zhang et al.‚ 2015). Cren7 and Sul7 can be methylated at several lysine residues (Guo‚ 2007). This PTM might be to regulate gene expression (Feng et al.‚ 2010)‚ although
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1. Sequence A TCT TCC CTC CTA AAC GTT CAA CCG GTT CTT AAT CCG CCG CCA GGG CCC CGC CCC TCA GAA GTT GGT Sequence B TCA GAC GTT TTT GCC CCG TAA CAA CTT GTT ACA ACA TGG TCA TAA ACG TCA GAG ATG GTC AAT CTC TTA ATG ACT Sequence C TAC AAA CAT GTA AAC ACA CCC TCA GTG GAC CAA CTC CGC AAC ATA AAC CAA ACA CCG CTC GCG CCG AAA AAG ATA TGG 3. 4. Sequence A - Middle Sequence AGA AGG GAG GAU UUG CAA GUU GGC CAA GAA UUA GGC GGC GGU CCC GGG GCG GGG AGU CUU CAA CCA Sequence B - End Sequence
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DNA STRUCTURE AND REPLICATION One may wonder how a single cell becomes two cells‚ and why this is ultimately important to life. For this essay‚ it would be much too difficult to discuss the process of cell division‚ which is the biological basis of life. That being said‚ this essay will examine more closely the precursor to cell division‚ also known as DNA replication. DNA replication is the process of copying a double-stranded DNA strand on a chromosome within a cell. The process‚ in its totality
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Isolated DNA Products Amplified Via Polymerase Chain Reaction and Cloned Biotechnology: DNA WPUNJ December‚ 2012 Abstract Isolated DNA from mouse‚ plants‚ and plasmid DNA were used for Polymerase Chain Reaction (PCR) for DNA amplification. The purpose of this experiment was to study the success rate or optimization of PCR of DNA‚ using both manual and kit methods. This set of experiments gives an insight to the relative difficulties associated with the optimization of a variety
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HEMOGLOBIN & MYOGLOBIN Protein Function HEMOGLOBIN: WHEN THE FIRST SUBUNIT OXYGENATES OR DEOXYGENATES THE FOLLOWING THREE SUBUNITS FOLLOW SUIT AND THE SHAPE OF THE HBG MOLECULE IS CHANGED. Oxygenated • R state (relaxed) • When O2 is present‚ it binds to the iron attached to each heme and tugs on it which in turn flattens the heme to a planar shape • The color of oxygenated blood is red (macroscopic) • Carried from the heart throughout the body by the systemic arteries Deoxygenated
Free Hemoglobin Red blood cell Blood