the role of covalent bonds and hydrogen bonds in the structure of DNA. The role of covalent and hydrogen are as follows. hydrogen bonds‚ being easy to break‚ allow the DNA to break so that copies can be made. Whereas covalent bonds keep the sugar and phosphate together‚ which allows proper placement and structure. 3. Relate the role of the base pairing rules to the structure of DNA. The bases in a strand of DNA relate to the base pairing rule due to the combination of GC
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Topic: Concepts of DNA Fingerprint and Forensic Analysis Table of Contents Introduction………………………………………………………………………………. 3 What is DNA Fingerprint...................................................................................................3 Forensic Science…………………………………………………………………………..4 DNA Fingerprint Methods Restriction Fragment Length Polymorphism (RFLP)…………………………………...4 Polymerised Chain Reaction (PCR)……………………………………………………...5 Short Tandem Repeats
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Chapter 9 DNA-Based Information Technologies Multiple Choice Questions 1. DNA cloning: the basics Page: 307 Difficulty: 1 Ans: C Restriction enzymes: A) act at the membrane to restrict the passage of certain molecules into the cell. B) are highly specialized ribonucleases that degrade mRNA soon after its synthesis. C) are sequence-specific DNA endonucleases. D) are very specific proteases that cleave peptides at only certain sequences. E) catalyze the
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box in a class 2 promoter. He got a piece of DNA that had a good class 2 promoter and a piece of DNA that he knew he could cut with restriction endonucleases. The idea is to pair a piece of DNA with label on only one strand. In this case you want the label on the crick strand. You would take this piece of DNA with a hot phosphate on 5 prime end of the crick strand and denature it. The probe DNA helps you see where txn starts. You take that probe DNA and you mix it with transcript thats made by the
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The DNA Identification Act of 1994 authorized the establishment of a national index of: (1) DNA identification records of persons convicted of crimes‚ (2) analyses of DNA samples recovered from crime scenes‚ and (3) analyses of DNA samples recovered from unidentified human remains. In addition‚ it specified several standards for those laboratories that contribute profiles to the national index system‚ including proficiency testing requirements for DNA analysts and privacy protection standards related
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Forensic DNA testing In Today’s forensic DNA testing is used all around the world to catch criminals. Forensic DNA testing is able to catch suspects and analyze their data correctly to be able to identify their suspect and be sure to identify their suspect and be sure it is the right person. DNA testing is 100% accurate and is the most effective type of testing there is and can change many lives such as criminals‚ fathers and children. Before forensic DNA testing
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sequencing; Highthroughput sequencing technology Canonical DNA: • • • • • Chemical structure and properties of B-DNA Enzymes that function on B-DNA DNA dissociation and reassociation kinetics of B-DNA Chromicity of B-DNA Major and minor grooves and DNA-protein interaction in gene regulation of B-DNA Non-canonical DNA: • Structure of A-DNA and Z-DNA; Comparison of A‚ B and Z-DNA; • Discovery of Z-DNA in vitro and in living cells; • Z-DNA and human genetic diseases Role of Major and Minor Grooves in Gene Regulation
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serial dilution of the DNA concentration. As shown by the picture‚ only the first drop of the DNA/EtBr mixture for the DNA standards fluoresce brightly under the UV light‚ while the other spots for both the DNA standards and the unknown DNA standards were all dimly fluoresce. This was due to pipetting error; the tip of the pipette did not touch the liquid (TE) in the micro-centrifuge‚ so no DNA standards were transferred into the liquid. In the first row (known DNA standards)‚ the DNA was inserted into
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Date: Student Exploration: DNA Fingerprint Analysis Vocabulary: codon‚ DNA‚ DNA fingerprint‚ genotype‚ identical twins‚ nitrogenous base‚ phenotype‚ trait Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. The two navy officers shown at left are identical twins. Why do you think identical twins look so similar? Identical twins look so similar because identical twins have the same genetic makeup‚ meaning‚ the same DNA (same nitrogenous bases). This is because
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Date: 09/21/2012 Result The purpose of the experiment is to measure the amount of DNA extracted from a strawberry. In the experiment‚ we measured the absorbance of different concentrations of Methylene Blue. The data collected by our group is presented on Figure 1 and the average absorbance measured by whole class is stated on Figure 2. Table 3 shows the measurement on the amount of strawberry DNA extracted. In Figure 1 and Figure 2‚ the data will be presented Standard Curve Graph. The
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