CHEM120 Week 7 iLab: DNA‚ mRNA‚ and Protein (30 points) Name: Kaylee Klefman Complete the two questions below. Each question has four parts. This assignment is two pages long. Question 1: For the following double-stranded DNA sequence‚ -CATTGACCGTAA- -GTAACTGGCATT- Answer the following questions: a) Assume that RNA polymerase will read the top strand of DNA as the “template” to synthesize mRNA. What will be the sequence of the mRNA synthesized? (3 points) The new mrna
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DNA typing was first used in Great Britain for law enforcement purposes in the mid- 1980s. It wasn’t employed in the United States until 1987. DNA profiling has changed forensic science. DNA technology has given police and the courts a means of identifying the suspects of rapes and murders. Today‚ the Federal Bureau of Investigation performs the bulk of the forensic DNA typing for local and state law enforcement agencies. In criminal investigations‚ DNA from samples of hair‚ bodily fluids or skin
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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DNA analyzing has been playing an increasingly role in the medical field. Genetic diseases’ rate is progressively growing but with the new technology‚ doctors are able now to reduce that high rate by using new invention. For example‚ with DNA analyzing doctors have the ability to reorganize the genetic disorder to make it better. Furthermore‚ now they can catalyze human body’s cells to produce a healing or therapeutic substance that could control the disease instead of fighting it. In another point
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Discovery of DNA Erwin Chargaff: Chargaff was an Austrian biochemist‚ he was best known for “Chargaff’s Rules” which lead to the discovery of DNA’s double helix structure. He immigrated to New York and secured a position as a research associate in the biochemistry department at Columbia University. Became a full-fledged professor in 1952. He used chromatographic techniques in his research. It was in the U.S.‚ 1950’s where he was able to make the crucial elements‚ in which lead to the formation
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two cyclists was guilty‚ 4 different forensic tests were conducted. Because genetic blueprints are unique‚ DNA is key when attempting to convict an individual. Since DNA testing is very sensitive‚ one must conduct these tests carefully. A technique called Polymerase Chain Reaction (PCR) is used to make millions of copies of the DNA without destroying it. Then‚ a restriction enzyme cuts the DNA into different fragments called reaction fragment length polymorphisms (RFLPs) at specific binding sites.
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With a new study on evolution‚ scientists have discovered that the mitochondrial genomes of birds have found two mutations within the mitochondrial genomes within fifty years. Within those fifty years it has shown two mutations within the mitochondrial genomes which can debunk the two percent of evolution per million years. By studying individual chickens Professor Greger Larson at Oxford University’s Research Laboratory for Archaeology‚ they have found two mutations in the mitochondrial genomes
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Can you expect to extract more DNA from a strawberry or kiwi fruit? Introduction: In the experiment we were inspecting the amount of genetic materials in fruits with different numbers of chromosomes. In the experiment‚ we mashed up a strawberry/kiwi fruit (See appendix 1 figure 2) added extraction butter and ethanol to extract the DNA. A chromosome is a long thread-like cluster of genes in the nucleus. DNA (deoxyribonucleic acid)‚ a self-replicating material which is present in nearly all living
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The year is 2067. This is a landmark year in DNA technology and its impact on human reproduction. The DNA technology that now exists has surpassed anything imaginable fifty years earlier. Historically‚ Gregor Mendel is credited with some of the earliest research into genetic modification‚ but the first successful transfer of genetic material was not reported in medical journals until 1997 (Rangel). Since then‚ DNA and human genetic research and modification has grown exponentially‚ with the past
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derivations for calculation of ATP yield due to the complete oxidation of different types of fatty acids Banda Venkat Reddy1‚ (Published In Indian Journal of Biochemistry And Biophysics) Abstract During complete oxidation of fatty acids‚ the electrons removed from fatty acids in different forms (FADH2 and NADH2) pass through the respiratory chain‚ driving the ATP synthesis. Generally‚ the total ATP yield due to the complete oxidation of fatty acids is calculated by sum of the ATPs obtained due to oxidation
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