BASIC BIOCHEMISTRY WEBQUEST General Webquest Directions: Put your name‚ date and block at the top. Find the answers by visiting the links found directly following the questions and reading ALL of the information based on these four macromolecules - carbohydrates‚ lipids‚ proteins‚ and nucleic acids. Type you response after each question. BASIC CHEMISTRY 1. Draw 2 separate carbon molecules both with four single bonds connected to hydrogen molecules. Next take away two hydrogens
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e. a‚ b‚ & c Question 2 1 out of 1 points Nucleotides on a single-stranded DNA molecule are connected by: Answer Selected Answer: phosphodiester bonds Question 3 10 out of 10 points The polarity of DNA synthesis is Answer Selected Answer: 5’-->3’ Question 4 1 out of 1 points "The ratio of adenine to thymine in DNA (is) __________‚ while the ratio of adenine to cytosine (is) __________:" Answer Selected
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DNA & RNA Jo Howell Biochemistry‚ Task 208.5.1 02-05 Western Govenor’s University Process of DNA Replication The double helix is unwound by helicase & each strand then acts as a template for the next strands Primase creates the DNA primer DNA polymerase III is the main copying enzyme DNA polymerase I removes RNA primers & replaces with DNA Single Stranded Binding Proteins are available to hold the unwound DNA strands in position & prevents premature annealing with another
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is the process of joining two DNA fragments or other molecules by a phosphate ester linkage with the action of enzyme. Ligation of DNA is process that involve the DNA of interest is inserted into the plasmid. This 3’-hydroxyl of DNA terminus are joined together with 5’-phosphoryl of another by phosphodiester bond. The ligation of DNA fragments usually performed by using T4 DNA ligase. All the reaction components such as ligation buffer‚ DNA insert‚ pGEM®-T‚ and T4 DNA ligase is mixed by gentle pipetting
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22 mortal cell lines.Telomeres is a repeated DNA sequence(TTAGGG) found at the ends of linear chromosomes that protect the ends of the chromosome from degradation‚ or telomere can be defined as DNA sequences found at the ends of eukaryotic chromosomes which maintain the fidelity of genetic information during replication. At birth as determined by terminal restriction fragment analysis‚ telomeres consist of about 15‚000 base pairs of repeated TTAGGG DNA sequences‚ which become shorter with each cell
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Updated March 2011 DNA‚ RNA structure and function Why are multiple copies of tRNA and rRNA genes needed? a. These RNAs are destroyed very rapidly. b. These RNAs are very stable. c. These RNAs are needed in large amounts. d. These RNAs do not benefit from the extra amplification step that occurs for protein-encoding genes. e. c and d If you treat mitotic chromosomes with solutions that extract histones‚ what happens? a. The chromosomes become more condensed
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Replication fork Scheme of the replication fork. a: template‚ b: leading strand‚ c: lagging strand‚ d: replication fork‚ e: primer‚ f: Okazaki fragments Many enzymes are involved in the DNA replication fork. The replication fork is a structure that forms within the nucleus during DNA replication. It is created by helicases‚ which break the hydrogen bonds holding the two DNA strands together. The resulting structure has two branching "prongs"‚ each one made up of a single strand of DNA. These two
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Franklin Stahl are two biologists who prove that DNA replication was semiconservative. At the time‚ many strong evidences from experiments using bacterial viruses had already convinced most scientists that DNA was the molecule of heredity; however they knew little about the DNA replication process. After the dimensionally accurate model building by Watson and Crick‚ it was clear that the process of replication and information distribution have to use the DNA from parent cell as template to achieve an
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December xx‚ 20xx To the Fellowship Selection Committee: I am writing in support of Janet Lerner’s application for an NSF Fellowship. Janet is genuinely a gifted student with great potential as a research scientist. I encourage you to give her your most careful consideration. Janet has been conducting an independent honor’s research project in my laboratory for the past year. Without a doubt‚ Janet is one of those rare individuals that comes along only once every few years; she is highly intelligent
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Transmission of Information by DNA and RNA (My name) December 2014 GRT1 Biochemistry Task 1 DNA Replication at the Biochemical Level • A DNA (Deoxyribonucleic Acid) molecule is in comprised of two strands of nucleotides. • When DNA replicates‚ the nucleotide strands are split and used as a template for a new strand. – The original strand (template) is called the parent strand – The replicated strands are called daughter strands. – A complete daughter DNA strand contains one strand from the parent
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