Chapter 9 DNA-Based Information Technologies Multiple Choice Questions 1. DNA cloning: the basics Page: 307 Difficulty: 1 Ans: C Restriction enzymes: A) act at the membrane to restrict the passage of certain molecules into the cell. B) are highly specialized ribonucleases that degrade mRNA soon after its synthesis. C) are sequence-specific DNA endonucleases. D) are very specific proteases that cleave peptides at only certain sequences. E) catalyze the
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For over a decade‚ there has been a controversial issue dealing with building a national DNA database. This issue has been brought up over the discussion of the actual database and what kinds of effects will come out of it‚ if it actually happens to go through. Some people think the database will be a force in crime fighting. Others think it is a violation of civil liberties. In the early 1900s‚ “fingerprinting‚” a new crime-fighting database‚ was developed. With the exception of identical
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Running head: DNA Evidence and Job Description/Ethics Name Course Tutor Date How would the environment affect this fragile evidence? II. Collecting Fingerprints from a Weapon Describe how you would collect a fingerprint from a weapon that could possibly have touch DNA on it as well. UNIT 9: Job Description for Latent Print Examiner Write a job description for a Latent Print Examiner. Latent Print Examiner Salary scale: Between $ 70‚000 and $
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sequencing; Highthroughput sequencing technology Canonical DNA: • • • • • Chemical structure and properties of B-DNA Enzymes that function on B-DNA DNA dissociation and reassociation kinetics of B-DNA Chromicity of B-DNA Major and minor grooves and DNA-protein interaction in gene regulation of B-DNA Non-canonical DNA: • Structure of A-DNA and Z-DNA; Comparison of A‚ B and Z-DNA; • Discovery of Z-DNA in vitro and in living cells; • Z-DNA and human genetic diseases Role of Major and Minor Grooves in Gene Regulation
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serial dilution of the DNA concentration. As shown by the picture‚ only the first drop of the DNA/EtBr mixture for the DNA standards fluoresce brightly under the UV light‚ while the other spots for both the DNA standards and the unknown DNA standards were all dimly fluoresce. This was due to pipetting error; the tip of the pipette did not touch the liquid (TE) in the micro-centrifuge‚ so no DNA standards were transferred into the liquid. In the first row (known DNA standards)‚ the DNA was inserted into
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Date: Student Exploration: DNA Fingerprint Analysis Vocabulary: codon‚ DNA‚ DNA fingerprint‚ genotype‚ identical twins‚ nitrogenous base‚ phenotype‚ trait Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. The two navy officers shown at left are identical twins. Why do you think identical twins look so similar? Identical twins look so similar because identical twins have the same genetic makeup‚ meaning‚ the same DNA (same nitrogenous bases). This is because
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Extract DNA from Anything Living |[pic] | |Introduction: [pic] Since DNA is the blueprint for life‚ everything living contains DNA. DNA isolation is one of the most basic and essential techniques in the study of DNA. The extraction of DNA from cells and its purification are of primary importance to the field of biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders‚ produce DNA fingerprints
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Title: DNA analysis Aim: a) Isolate and Purify Bacterial Chromosomal DNA from a strain of E.coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E.coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different
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store your DNA in a database? Not only that‚ how would you feel if you found out that your DNA was in the wrong hands? The boundary between violations of individual privacy can be quite difficult to identify due to a variety of reasons. DNA is the hereditary material that is the fundamental building block for an individual’s entire genetic makeup; it can be used in order to determine a wide range of information about an individual (Wall‚ 2005). DNA test banks are the storing of DNA information
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Since its discovery in the 1980’s‚ the application of DNA has been utilized in many ways. When Deoxyribonucleic Acid‚ or DNA is examined‚ a precise decoding of genetic information is revealed. With the exception of identical twins‚ every person’s genetic code (commonly referred to as a Genetic Fingerprint) is inherited and unique. From Maury advising men everyday on TV “You ARE the Father!” or “You are NOT the Father!” to learning the descendents of former slaves are related to President Thomas Jefferson
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