The universal DNA database is important to the law enforcement agencies. The database will help the law enforcement agencies to identify suspects fast. When the universal data base is not used‚ it is very easy for a criminal to commit a crime and escape. The DNA universal database will improve the crime investigation thereby curbing the menace of criminals. When this takes effect‚ criminals might be extremely careful not to leave any trace that can help in getting their DNA for identification (Krimsky
Premium DNA Crime Police
BIO 219 Group 1 Section 66 October 19‚ 2012 The extraction of purified DNA from A. fischeri by restriction digestion using Sal I enzyme and pGEM for shotgun cloning Introduction: The ultimate goal of this experiment is to isolate the lux operon‚ a targeted piece of DNA that causes bioluminescence‚ from Aliivibrio fischeri and insert it into the DNA of Escherichia coli in order to make it glow. A. fischeri is a gram-negative bacteria which participates in a symbiotic relationship with
Premium DNA Escherichia coli Bacteria
We placed the lambda DNA into three test tubes. We then incubated one sample with EcoRI‚ one with HindIII‚ and the other we left as a control. After we completed the experiment and ran the DNA‚ we found that the first band of the crime scene DNA traveled 3.5mm and the second band traveled 6.5mm. The actual size of the first crime scene band is 1‚100bp and the second band is about 5‚500bp. The first band of suspect 1 DNA traveled 3 mm and the second band traveled 6.5mm
Premium Bacteria Antibiotic resistance DNA
abundant and versatile macromolecule‚ is made up of a chain of amino acids connected by peptide bonds. Carbohydrates are sugars made up of monosaccharides connected by glycosidic bonds. Nucleic acids are made up of nucleotides‚ and examples include DNA and RNA. Each tissue in the body has a different amounts of the four macromolecules. Based on a study done by Baker‚ the percentage of lipid in adipose tissue in humans at birth is about 40% and increases up to about 75% with age (1). According to
Premium Metabolism Protein DNA
bactaria was isolated and cloned. The expressed ldhA gene was inserted into pET28b plasmid vector. The resulting recombinant pET28b-LdhA expression vector was then transformed after introduction into E. coli. The ligation gave 15 colonies of recombinant DNA which later gave 6 recombinant plasmids as revealed by gel electrophoresis. 1. Introduction The NADH-dependent lactate dehydrogenase (EDH) is a key enzyme in the fermentative metabolism
Premium DNA Molecular biology Bacteria
It is when a bacterial cell takes up DNA that is foreign to it and integrates it into its own DNA. What is genetic transformation? That is change that is caused by genes involving the insertion of a gene into an organism to change the organism trait. This transformation typically occurs in plasmids. So‚ what are plasmids? Plasmids are small circular DNA molecules that are isolated from its chromosomes. So‚ what are genes and plasmids and how are
Premium DNA Gene Bacteria
Brianna Noriega In order to analyze a dna molecule in a molecular biology lab you must determine the length in nucleotide pairs. Electrophoresis is an extremely useful tool in order to compare the mobility on agarose gels with dna markers of known lengths. Dna is a polymer that is negatively charged due to the sugar phosphates. When dna is on an electric field such as the electrophoresis gel the different lengths of dna migrate at different rates when they move through the porous gel. The ends
Premium DNA Molecular biology Protein
qualify this paper either in 1st or 2nd or 3rd year examination. (9:00 a.m. to 10:45 a.m.) 7th‚ Monday --------- Bio-informatics & Environmental Microbiology EVENING --------- Recombinant DNA Technology and Bio-Analytical Techniques BMF-2001: Microbial Genetics & R-DNA Technology (1316) Environment and Road Safety Education (0353)
Premium Food processing DNA Biotechnology
Experiment: DNA damage and mutations in human cells when exposed to nitric oxide Aim: To examine mutations after in vitro exposure of nitric oxide to human cells. Abstract: Nitric oxide (NO) is a anatomical carrier formed by various cell types. In this experiment nitric oxide is made to react with undamaged human cells and solutions of DNA‚ RNA ‚ guanine or adenine in aerobic conditions. TK6 human lymphoblastoid cells were altered. It has been observed that deamination of purines‚ pyrimidines
Premium Nitric oxide Oxygen DNA
Purpose of the lab: The pupose of the lab was to transfor a bacterial E. Coli by using the green flurescent protein from the jellyfish. Another important that was fferdone by making the cell competency‚ meaning that it will be able to take on additional DNA. This was done when the plasma was added. Materials: 1. 37 o C water bath 2. Ice 3. Sterile transfer pipette 4. Foam tube rack 5. Transformation solution (CaCl2) 6. pGLO plasmid 7. Sterile Inoculating loop 8. 2 - LB+amp plate 9. LB+amp+ara plate 10
Premium Bacteria Escherichia coli DNA