Specimen Preparation: Samples and all test reagent and kit were brought into room temperature. In 2ml eppendrof tube approximately 220mg stool sample were taken. Washing buffer was prepared by adding distilled water . Procedure for Purification of DNA from Stool sample: i. 220mg stool sample were collected in a 2ml tubes and placed it on ice. ii. Added 2ml Buffer ASL to each stool tube. Used pipet to wash the stool sample from the spoon while transferring the buffer. Vortexed continuously for 1minute
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DISCUSSIONIn the first part of the lab‚ since the effect of moist heat treatment on bacterial spores wasbeing investigated‚ the spores from Bacillus stearothermophilus was used. Using the three testtubes with spores‚ the first one (1) was autoclaved‚ the second (2) was boiled‚ and third (3)received no treatment. The fourth test tube (4) didn’t have a spore strip and served as a controlfor the effectiveness of the aseptic pipetting technique. As hypothesized‚ test tubes 1 and 4experienced no bacterial
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business report and a scientific report on the basis of Yeung´s article “In search of commonalities: Some linguistic and rhetorical features of business reports as a genre”. Traditionally business reports are taught mainly based on the model of scientific reports which means they have lots of similarities. Both are using a standardized format‚ consisting of summary‚ introduction‚ objectives‚ methods‚ results‚ discussions‚ conclusions and recommendations and as well as a scientific report a business
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March 3‚ 2013 Wrongful convictions. | How the use of DNA can exonerate those wrongfully convicted. Imagine wasting years of your life in a jail cell on death row‚ for a crime you did not commit. You have to ask yourself “how could this happen? How did an innocent person get convicted if indeed they are innocent?” Those are just a few questions you think of when you think of wrongful convictions. Some questions can be answered by the common causes of wrongful convictions‚ such as‚ eyewitness
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Discuss the legal implications of the use of DNA evidence in the NSW criminal justice system DNA evidence is a widely used tool in the NSW criminal justice system that aims to help achieve justice. DNA‚ short for deoxyribonucleic acid‚ is a long molecule found within the cells of the human body. Each cell contains genetic material in which‚ apart from identical twins‚ is exclusive to every individual. DNA though considered a reliable piece of evidence can present many issues in the criminal
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Name: Jacob Diaz Sequence: CCCCTGCTGGGAGTGGGGCTGAACACGACAATTC Sequence ID/Fragment Code: 6649013 Answers: 1. Identify the gene from which the query sequence originates (Name of gene) - Homo sapiens interleukin 2 receptor‚ gamma (severe combined immunodeficiency (IL2RG)‚ mRNA - See Appendix 1 2. Provide the full protein sequence encoded by the gene. - >gi|4557882|ref|NP_000197.1| cytokine receptor common subunit gamma precursor [Homo sapiens] MLKPSLPFTSLLFLQLPLLGVGLNTTILTPNGNEDTTADFFLTTMPTDSLSVSTLPLPEVQCFVFNVEYM
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The Relationship between Gene Copy Number‚ Amylase Concentration‚ and Gene Evolution Matthew Fantauzzi 400007178 Shawn Hercules - L15 25 November 2015 Abstract In this lab‚ students were experimenting to determine if a relationship exists between gene copy number‚ amylase concentration‚ and gene evolution. At the same time‚ this lab was designed to introduce university freshman to the etiquette and conventions used in a formal research setting. The methods used ranged from sample production
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involved in facultative heterochromatin stabilization. Chromatin‚ made up of DNA and associated proteins‚ can be euchromatic or heterochromatic; euchromatin is mainly associated with active transcription‚ whereas heterochromatin is associated with repressed transcription. Active euchromatin and repressive heterochromatin are established by post-translational marks such as methyl groups placed on the histone around which DNA is wrapped. Histone 3 lysine 4 is methylated to promote active transcription
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chemical mutagen‚ Ethyl methane sulfonate (EMS)‚ and a technique that identifies single base changes within the target gene. With the TILLING method‚ multiple alleles are amplified by PCR to for DNA heteroduplexes which are double stranded nucleic acid. When it is heated and cooled‚ a bubble forms where two DNA strands are mismatched and is cleaved by single stranded nucleases. Mismatches can be because of induced mutation or natural variation (Henikoff‚ Till‚ & Comai‚ 2004). The cleaved products are
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Bita Heydari Lab report 3 The Effects of Differentiation on Enzymatic Activity Introduction HL-60 cells are capable of undergoing differentiation to induce different cell types. HL-60 cells can undergo morphological changes‚ changes in gene expression‚ and changes in protein synthesis. In the past weeks‚ we were able to conclude that HL-60 cells treated with DMSO and HL-60 cells treated with PMA will differentiate into granulocytes and monocytes upon treatment (1). We were also able to observe
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