was used to obtain colonies which were resuspended into a CaCl2 solution that was previously kept on an ice bath. The rainbow transformation mixture containing the plasmid DNA was then added to half of the E. coli cells. These cells were later placed into a water bath set to 42ºC and “heat shocked” to promote the entrance of DNA into the cells. Moreover‚ a Recovery Broth was added to the sample and the sample was left undisturbed for 30 minutes at room temperature so the “heat shocked” bacteria would
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Through endocrine signaling‚ the signaling molecule or hormone is produced by a gland and discharged into the blood where it travels until it reaches certain blood cells distant from its place of origin on which it exerts its action. This is a long distance communication process. The signaling molecule is mainly of protein origin‚ although there is also a varied range of steroidal hormones. (ENDOCRINE SIGNALLING: Image from University of Kent https://moodle.kent.ac.uk/external/mod/book/view
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Meiossis and Genetic Diversity in Sordaria Meiosis and Genetic Diversity in the Model Organism‚ Sordaria. By: Katie Fiorillo BIO110 Lab TA: Lauren Smith October 30‚ 2012 A. INTRODUCTION: In this lab‚ the organism Sordaria fimicola was used to explore factors contributing to genetic evolution of sexual reproduction. These organisms are found in “Evolution Canyons‚” which occur when two mountain slopes with very different climatic conditions congregate with a comparatively small valley
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Introduction The purpose of this lab is to understand how each offspring inherits its genotype and how its phenotype reflects it. Sex chromosomes determine the sex of the offspring‚ and the autosomes consist of all the other chromosomes that determine phenotypic traits. Dominant traits are the genes that are expressed‚ while the recessive traits are masked by the dominant traits. We used Drosophila melanogaster (fruit fly)‚ mutating the female fruit fly with a scalloped gene to change the phenotype
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Through this experiment‚ Egeria densa was observed using a microscope. The task was to observe and identify the different types of cell‚ cytoplasmic streaming‚ and plasmolysis of Egeria densa. First‚ the microscope was examined and investigated to master the use of the equipment. A microscope slide grid which was on the slide glass was required to be seen clearly using 4x‚ 10x and 30x. During the latter part of the experiment‚ the Egeria densa was observed using the microscope to understand the
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Question: What antibiotics work best in preventing E. coli k12 from growing; amoxicillin‚ ampicillin‚ or ciprofloxacin? Hypothesis: If ciprofloxacin‚ an antibiotic‚ is added to petri dishes covered in live bacteria and left to sit for four to six days in an incubator at 37 degrees Celsius‚ then the petri dishes containing ciprofloxacin will have the largest zone of inhibition out of all of the other antibiotics tested because research shows that ciprofloxacin in one of the strongest antibiotic currently
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Title: E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation
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Purpose In this lab‚ we used PCR and gel electrophoresis to identify genetically modified food. Introduction A genetically modified organism is an organism whose DNA or genetic makeup has been modified to code for certain desirable traits("Genetically Modified Foods"). Common genetically modified plants include corn and soy‚ and common genetically modified animals are fish. Many genetically modified plants are coded to resist bugs‚ grow faster‚ and produce bigger fruit‚ while most GMO animals
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Did you know that all organisms consist of basic units of matter called atoms. When atoms are bonded together they make up a molecule. Often associated with nonliving things are inorganic molecules‚ and associated with organisms are biomolecules. Today in lab‚ a series of tests were ran. Tested were the biomolecules of cells which are lipids‚ sugars‚ proteins and starch. The purpose of these tests were to test for the presences of cellular biomolecules. Some of the things that fat consist of are
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Polypeptides‚ better known as proteins‚ carry out nearly all of a cell’s functions. A protein is made up of at least one or more amino acid polymers. The necessity of proteins for life is unequivocal‚ not only do they help to form the structures of cells but they act as enzymes in reactions of the body1. Enzymes are biological catalysts that lower the amount of activation energy needed in carrying out biochemical reactions1. Enzymes are responsible for almost every reaction that occurs in a cell
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