Procedures: In the first lab‚ seven test tubes were attained and six of them were filled with the solutions that were listed (Na Pyruvate‚ MgSO4‚ NaF‚ Glucose‚ Water‚ and yeast suspension). The last test tube was filled with water. After they were filled with the solutions they were incubated at 37 degrees Celsius for about forty minutes. After the forty minutes passed take the test tubes and measure the height of the bubbles that formed in millimeters. For the second lab‚ attain three beakers‚
Free Carbon dioxide Oxygen Carbon
is associated with lactase persistence is the located upstream of the LCT gene. In this mutation a single nucleotide polymorphism changes a cytosine into a thymine that then can be detected using the polymerase chain reaction technique (Biology 225 lab manual‚ S2017). In this experiment amplification Refractory Mutation System PCR was used to detect this nucleotide change. Two primers were used in two different PCR reactions‚ one to detect the wild type allele and the other to detect the mutant allele
Premium Glucose Carbohydrate Sugar
Egg Lab Report Introduction: An egg is a model of a human because the egg has a cell membrane like humans do inside and outside of the body that let things pass through like water. We can use eggs to study the effect of changes in the external environment on the internal environment by having harsh environments like putting the egg in only alcohol and see what happens to the inside of the egg. Diffusion is the movement of a substance down its concentration gradient from a more to a less concentrated
Premium Osmosis Diffusion Water
one outlier that was strange was the one paper towels. Only 14 of the 15 seeds germinated in that group. The difference that we could observation was the healthiness of the plants. IV. Conclusion Paragraph 1 Recap (Evidence): The purpose of our lab was to see if the amount paper towels would affect the germination of the seeds. My hypothesis was‚ if I increase the amount of paper towels‚ then the germination rate would increase too. We observed the petri dishes in class and we would add 2 ml of
Premium Seed Germination Plant morphology
The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
Premium Laboratory glassware Laboratory equipment Chemistry
Abstract The aim of the experiment is to test the existence of macromolecules which are carbohydrates‚ lipids‚ nucleic acids and proteins in given samples. The first experiment is to test the existence of carbohydrates in starch suspension and potato cell. If carbohydrate is present in starch suspension‚ the solution will turn into dark blue when iodine solution is added. If carbohydrate is present in potato cell‚ starch granules can be seen clearly under microscope when iodine solution is added
Premium Starch Carbohydrate Chemistry
Introduction In this lab‚ the purpose was to verify Hess’s Law. Four main topics were covered during this experiment including enthalpy of reaction‚ heat of formation‚ Hess’s Law‚ and calorimetry. The enthalpy of reaction‚ ΔHrxn is the heat or enthalpy change for a chemical reaction. The energy change is equal to the amount of heat transferred at a constant pressure in the reaction. The change represents the difference in enthalpy of the products and the reactants and is independent of the steps
Free Enthalpy Thermodynamics Temperature
are many conditions of the environment that can affect the optimum operation of enzymes. These condition include temperature‚ enzyme concentration‚ substrate concentration‚ acidity‚ salinity‚ and any present activators/inhibitors. In this particular lab‚ temperature was the environmental factor studied. More specifically‚ the enzyme catalase and its substrate hydrogen peroxide were tested under different temperatures. It was discovered that‚ temperature can affect the optimum operation of enzymes;
Premium Enzyme Metabolism Catalysis
According to the data from both the lab group and the class average‚ there is evidence that osmosis did occur in the bags. The largest change in mass was in the 1.0M sucrose bag the mass went from 12g initially to 14.2g‚ this gained 2.2g‚ an 18.3% change in mass for the group data over the duration of the experiment. The 0.2M bag went from 10.2g to 10.9g a 6.9% change in mass; the 0.4M bag went from 12.1g to 12.2g .83% change in mass; the 0.8M bag went from 10.9g to 12.2g and an 11.9% change in
Premium Chemistry Concentration Osmosis
Lab Report Part II Purpose: To be familiarized with the science and techniques used to identify different types of bacteria based on their DNA sequences. Background Information: The process begins with preparing a sample. Successful identification starts with using a sample that is considered to be good. The first step is to pick up a single colony and drop it into a microcentrifuge tube. A buffer is used to dissolve the cell wall in order to extract bacterial DNA. This step may take several hours
Premium DNA Polymerase chain reaction DNA replication