Objective: The purpose of this lab is to allow you to become familiar with a procedure for extracting DNA‚ collecting DNA samples‚ and to observe the physical characteristics of DNA Background: The wild strawberry is a diploid because it only has two sets of chromosomes. While the grocery store kind is an octoploidy because it has eight sets of chromosomes. The reason we chose the grocery store strawberry is because we’d be able to extract more DNA. Ripe strawberries make enzymes which help break
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DNA Extraction Lab Problem Statement: Do you think you have ever eaten DNA? Background Information: DNA is too small to see under a regular microscope‚ so how can it be studied? DNA is a large molecule found in all living things; therefore it is possible to extract it from cells or tissues. All we need to do is disrupt the cell’s plasma membrane and nuclear envelope‚ make the DNA clump together and - voila! - DNA extraction is possible. DNA extractions from onion‚ bananas‚ liver‚ or wheat
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Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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DNA TECHNOLOGY How is DNA technology used in law enforcement? There are many different ways it is used‚ but it is mostly used to solve crimes. Much like deaths‚ rapes‚ or even big robberies. DNA is a way to identify a person by: blood‚ hair‚ skin‚ saliva etc... That is why when people are trying to discover a murderer they are very careful when they are collecting evidence.That is why when something occurs‚ everything is blocked off‚ and only investigators could pass into the area
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Strawberry DNA Extraction Donovan Roberts Mrs. Caudill Honors Biology 3/7/11 Introduction The structure of DNA is made of nitrogenous bases‚ phosphate groups‚ and sugars. These three parts of DNA form hydrogen bonds and create a right-handed double helix. The location of DNA in a eukaryotic cell is found within the nucleus‚ bound to proteins. DNA can be extracted from within the cell. They are several steps you must take to retrieve DNA from within the cell. When extracting DNA cells can
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DNA Technology in Crimes Is there a better way of solving crimes with DNA evidence to help investigators? The use of DNA technology is the best way to solve investigations. DNA makes it possible to identify people and to know who was at the crime scene based on a little piece of evidence. It helps solve crimes‚ and even‚ open cold‚ unsolved cases. DNA technology in criminal investigations has become an essential tool because of its ability to identify culprits through the use of fingerprints‚ blood
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Technology based laboratory to improve science learning Abstract Till date‚ manual routine devices and methods are used to perform different test strategies in the greater part of the science research centers in our nation. The manual instruments are cumbersome though the robotized ones are expensive. It does not enthuse youthful analysts towards the science laboratories. There is a need to create applications which can be easily integrated‚ customized in school and undergrad level laboratories
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Using DNA in Science and Technology DNA is the blueprint for life‚ it is found in the nucleus of cells on the chromosomes. It is found in many cell organelles‚ such as plasmids in bacteria‚ chloroplasts in plants‚ and mitochondria in both plants and animals. DNA helps us to perform many actions in solving crimes‚ and also helps us to do scientific studies that were not possible until recently. DNA has a double helix structure‚ its sugar phosphate backbone‚ made up of deoxyribose and a phosphate
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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