DNA profiling DNA profiling (also called DNA testing‚ DNA typing‚ or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals on the basis of their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a person’s DNA makeup‚ which can also be used as the person’s identifier. DNA profiling should not be confused with full genome sequencing. It is used in‚ for example‚ parental testing and rape investigation
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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OCEAN COUNTY COLLEGE OFFICIAL COURSE DESCRIPTION DEPARTMENT OF SCIENCE AND ENGINEERING 1. Course Number and Title: CHEM-181 General Chemistry I 2. Semester Hours: 4 Contact Hours: (3 + 2) Lecture Lab 3. Catalog Description This course‚ intended for science majors‚ is the first course of a two-course sequence. Course topics include stoichiometry‚ inorganic nomenclature‚ solutions‚ gas laws‚ thermochemistry‚ atomic structure‚ and chemical bonding. The laboratory work includes
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Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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DNA extraction in Watermelon and Bananas Abstract During this experiment the researcher investigated the differing amounts of DNA present in bananas and watermelon. So that the researcher could do this‚ and accurately record the amounts‚ the researcher liquefied the fruits in a blender‚ added water and salt to them‚ and the added laundry detergent before using contact solution as an enzyme. Rubbing alcohol was then poured into the mixture and the DNA was extracted and
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larger. Disk storage technology in general has seen incredible advances including reduction in size‚ an increase in real density‚ and an increase in internal data rates‚ but the future of hard disk technology is uncertain. Digital production‚ transmission and storage have revolutionized how we access and use information but have also made archiving an increasingly complex task that requires active‚ continuing maintenance of digital media. This challenge has focused some interest on DNA as an attractive
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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