double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this has played a crucial role in the exponential advancement of biotechnology in recent decades. Restriction enzymes cut DNA at specific
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have made the use of DNA in forensic science possible. In the past twenty years specifically‚ there have been many extraordinary discoveries in the fields of science that have led to the advancement of procedures in forensics. Before DNA testing‚ the most accurate way of identifying people was to match the blood types of suspects with blood found at the scene of the crime. Considering the lack of variability of this procedure‚ it is no surprise just how important the use of DNA in forensics has become
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Introduction In 2015 Congress passed the DNA Fingerprint Act‚ which required that‚ beginning January 1‚ 2009‚ any adult arrested for a federal crime provide a DNA sample. As of May 2013‚ 29 states‚ in addition to the federal government‚ have enacted arrestee DNA collection laws‚ which authorize collection of DNA following arrest or charging. A U.S. Supreme Court decision‚ Maryland v. King (2013)‚ upheld a Maryland state law that allowed for the warrantless collection of a DNA sample for those arrested for
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WHAT IS RECOMBINANT DNA So what is rDNA? It stands for Recombinant DNA. Before we get to the “r” part we need to understand DNA. DNA is the keeper of all the information needed to recreate an organism. All DNA is made up of a base consisting of sugar‚ phosphate and one nitrogen base. There are four nitrogen bases‚ adenine (A)‚ thymine (T)‚ guanine (G)‚ and cytosine (C).The nitrogen bases are found in pairs‚ with A & T and G & C paired together. The sequence of the nitrogen bases can be
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DNA Replication Deoxyribonucleic acid (DNA) contains the genetic instructions for the biological development of a cellular form of life including some viruses. DNA is an antiparallel double helix molecule with sugar-phosphate backbone on the outer side and nitrogen bases in the inner side. The bases are paired specifically‚ also known as complementary pairing‚ Adenine (A) with Thymine (T)‚ and Guanine (G) with Cytosine (C) by two and three hydrogen bonds‚ respectively. DNA is a long polymer
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DNA REPLICATION WHAT IS DNA? DNA is a molecule that has a repeating chain of identical five-carbon sugars (polymers) linked together from head to tail. It is composed of four ring shaped organic bases (nucleotides) which are Adenine (A)‚ Guanine (G)‚ Cytosine (C) and Thymine (T). It has a double helix shape and contains the sugar component deoxyribose. THE PROCESS OF DNA REPLICATION How DNA replicates is quite a simple process. First‚ a DNA molecule is "unzipped". In other words‚ it
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DNA Lab Report SungYong Jang What is DNA? What do the letters stand for? What is it composed of? Where is it found? What is it shaped like? Answer in full sentences. It is the genetic material that can be passed on from parent to offspring DNA --> Deoxyribonucleic acid It composed of Deoxyribose (5 carbon sugar)‚ a phosphate and a Nitrogonous base (the 4 N-bases are Guanine‚ Cytosine‚ Adenine and Thymine) It is a double helix (looks like
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EXPERIMENT NO. 1 AIM: To perform isolation of the genomic DNA from the bacterial cell. REQUIREMENTS: Biological: Bacterial culture (DH5α) Chemical: Solution 1 - 10ml • Glucose (50mM) - 500μl • Tris-Cl (pH 8.0‚ 25mM) - 250μl • EDTA (pH 8.0‚ 10mM) - 200μ Solution 2 • SDS (1%) Phenol: chloroform: isoamylalcohol (25:24:1)‚ Absolute ethanol‚ 70% ethanol‚ Sterile distilled water Apparatus: Micropipettes‚ conical flask‚ measuring
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DNA is the common name for Deoxyribonucleic acid. This is made up of nucleic acids containing deoxyribose (sugar)‚ consisting of complex molecules‚ present in the chromosomes of all plant and animal cells‚ and carrying in coded form instructions for passing on hereditary characteristics. The DNA molecule takes the shape of a double helix‚ a simple structure that resembles gently twisted ladders. The rails of the ladder are made of pairs of nitrogen-containing nucleotides‚ which are subdivisions
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plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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