The experiment did not contain any form of reaction mechanism since no chemical identity had been changed. The lab demonstrates the use of chemical molecular behavior to isolate a particular set of molecules. Caffeine had already existed in the leaf itself but needed to be separated from the other chemicals. Caffeine’s chemical structure is relatively similar to the nucleic acid purine in that they use nitrogen and is bicyclic but lacks an alkene‚ amine and an amide. Caffeine has a solubility of
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The objective of these labs were to use sophisticated techniques to produce double-stranded RNA that was incubated with live Drosophila cells to inhibit the expression of our two genes of interest. The overall process of the four labs was to isolate and amplify DNA using the polymerase chain reaction with primers that contained gene-specific sequences to Thread or Dynamin-related protein 1 (drp-1)‚ along with T7 promoter site sequences. The amplified DNA was purified and both strands of the DNA
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Enzymes Reactions to Changes in Substrate and Inhibitors Benjamin J. Mora Coronado University of Texas Rio Grande Valley at Edinburgh Abstract Purpose for the experiments was to test the enzymes in various scenarios and see how changing this would affect the rate of reaction. The enzyme source used in the experiments was Turnip Extract. Concentrations of Turnip extract for activity 1 where o.5ml‚ 1.0ml‚ and 2.0 ml as for the rest of the activities 2 Through 4 stayed at a consistent concentration
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From this lab we can learn to observe physical properties of varying substances as well as to use chemical reactions to observe chemical observe the chemical properties of varying substances. In experiment #1‚ we were able to detect physical properties of the aluminum and copper sulfate simply by looking at the substances. We then combined the two substances to see if they would create a chemical reaction and test the chemical properties. After being combined‚ bubbles and gas were created and the
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Andrea Negrete Abraham Lincoln High School Period 5 1/12/15 1/20/15 Partners: Nasya Aguilar LAB 1: Kinematic Equations and Reaction Time PURPOSE/QUESTION Apply kinematics equations for constant acceleration to find your reaction time. How much is it? How does reaction time change with practice? THEORETICAL The reaction time is the amount of time required to sense astimulus‚ analyze its meaning‚ and respond. Acceleration is the rate of change of velocity. Velocity is speed with direction
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The Diels-Alder Reaction Submitted by: Joe Student Partner: Jill Student Date: February 8‚ 2005 TA: Super TA Reaction +OOOxylene140 oCOOO Data Table Reagents MW Grams used Lit. Mp Anthracene 178.23 g/mol 1.023 g 216-218ºC Maleic anhydride 98.06 g/mol 0.497 g 54-56ºC Products MW Grams obtained Lit. Mp Observed Mp Crude product 276.29 g/mol 1.362 g 261-262ºC 260.2-261.3ºC Recryst. Product 276.29 g/mol 1.283 g 261-262ºC 261.3-261.9ºC Results and Discussion Discuss the
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Temperature and Cofactors on Enzymatic Reactions “I pledge that no unauthorized assistance has been given or received in the completion of this work. Experiments described were performed by me and/or my lab group and this write-up is entirely my own creative work.” X________________________________________ Introduction Enzymes are protein molecules that speed up the rate of reactions by reducing the activation energy of a reaction. They act as catalysts in reactions‚ increasing the rate at which
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The SN2 reaction requires three qualities: a strong nucleophile‚ a good‚ unhindered leaving group‚ and a polar‚ aprotic solvent. For our reaction‚ we have all three bases covered. The nucleophile is an alkoxide‚ a deprotonated alcohol. Technically‚ because our alcohol is a phenol‚ the conjugate base is called a phenoxide. Phenol itself has a pKa of about 10‚ but our alcohol has more resonance opportunities‚ so the pKa is down around 8. This is sufficiently acidic for use of weak base like K2CO3 for
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Title: Redox Reactions Research Question: Is it possible to determine if a redox reaction took place by using only the equation? Hypothesis: Yes‚ I believe it is possible to determine if this reaction took place by using the oxidation numbers in the equation. Variables: 1. I cleaned the reaction surface to limit contamination 2. I held the chemicals at approximately a 90 degree angle for consistency Introduction: Redox reactions occur when both oxidation and reduction take place. All atoms
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Calculations CALCULATION IN ORDER TO FIND THE PERCENTAGE OF VITAMIN C Chemical reaction: C6H8O6 + I2→ 2I + C6H6O6 Ascorbic Acid: C6H8O6 Relative formula mass of C6H8O6= (12.01076) + (1.007948) + (15.99946)= 176.12412 g/mol Convert Iodine lost from mL to dm-3 = Iodine lost in mL1000= Iodine lost in dm-3 Convert Iodine lost (dm-3) to moles (n) by multiplying it with the concentration of Iodine used: n=0.005 Iodine lost in dm-3= mol of C6H8O6 Find the mass (g) of C6H8O6 in 50 mL by using this
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