LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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independently of each other. Apply the rules of probability to infer genotypes from test crosses A test cross is used to determine the genotype of an individual with a dominant trait. Because the trait is dominant‚ an individual with the trait could be homozygous or heterozygous for the trait. (This cannot always be determined by simply looking at the phenotype of the individual.) In a test cross‚ an individual with the dominant phenotype is crossed with a fully recessive individual Define the concepts
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EXPERIMENT NO. 1 INTRODUCTION TO LAB INSTRUMENTS. 1. BREADBOARD We should be familiar to the following things about a breadboard: * What is a breadboard and what is it used for? * How does it work? * Setting Up. * Limitations. What is a breadboard and what is it used for? A breadboard (or protoboard) is usually a construction base for prototyping of electronics. The term "breadboard" is commonly used to refer to a solderless breadboard (plugboard). It was designed by
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chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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Part 2: Safe work with large inductances 1. One of the main hazards of using inductances is electrical energy stored in the magnetic field. If a circuit containing an inductance is interrupted then the stored energy can release through air (forming an arc). This can destroy a switch contact‚ burn fingers‚ create other damage. In the circuit of Figure 1‚ show how to mitigate the risk (pay attention to polarity!). We give the elements in this circuit some simple values for the purpose of
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Title Simple‚ no more than 20‚ 15 ample Cross cultural drinking experience in 1st year students. Abstract Summary of the research: 100-150 words Introduction‚ method (drop of the design)‚ Results (inferential statistic‚ Why is it significant)‚ Discussion. Compose at the end. Introduction About 800 words Introduces the area theorem or topic etc. Evidence (pull out the criticism) Rationale H1: Extroverts have a higher pain threshold than introverts H0: Extroverts do not have a higher
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concentrations of solutes in and out of the cell. Filtration is what is used to remove solid particles and they can be removed by passing through a liquid and gas. The information above is very important because it is exactly what everything in this lab will be about. It explains in detail what every single little definition and important
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BACK TITRATION- DETERMINATION OF THE CARBONATE CONTENT IN GARDEN LIME NAME: OSEI BONSU ERIC ID: 3906409 EXPERIMENT: I.2.2.1.
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