Buffer Preparation (Gozani Lab) 1. 1 M Tris-HCl Buffers pH Volume (L) TrisBase (g) HCl (ml) pH 7.0 2 242.2 150-155 pH 7.5 2 242.2 120-125 pH 8.0 2 242.2 80-85 Autoclavable. 2. EDTA 0.5 M (pH8.0) 0.5M‚ 1L: 148 g EDTA + ~30-40 g NaOH to adjust pH (or 186 g EDTA-Na.2H2O + ~20 g NaOH) Note: pH adjusted by NaOH is essential for solubility. Autoclavable. 3. TAE DNA Electrophoresis Buffer (50 X) (2 M Tris‚ 50 mM EDTA) 2L 484 g Tris 114.2 ml glacial acetic acid 200 ml 0.5 M EDTA 8.0 To make
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A buffer solution can maintains a narrow range of pH even when strong acid are added. In our experiment‚ we can see water is not a buffer‚ water can undergo very big changes when small amounts of strong acid or strong bases are added. When the strong acid‚ 100µl HCl was added into the dd water‚ the pH value changed from 7.38 to 5.83 which mean the dd water has turn into acid. The same phenomenon occurs while strong base‚ 100µl NaOH was added into the dd water. The pH value changed from 7.07 to 9
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BUFFER SOLUTION (ACID) An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts - often a sodium salt. Objectives: 1. Measure the pH of the unknown solution first with indicators and then with pH paper. 2. compare the buffer solution with both a strong acid and a weak acid materials: 2 30 mL beakers 2 100mL volumetric flask 1 10mL pipet 2 aspirator 1 50mL graduated cylinder 1 10mL graduated
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Buffer systems * prevent major changes in pH of body fluids by removing or releasing H+ * act quickly to prevent excessive changes in H+ concentration. Body’s major extracellular buffer system is “bicarbonate-carbonic acid buffer system”. There are 20 parts of (HCO3) to one part of (H2CO3). ---- [20:1]. When the ratio is no longer maintained‚ it will result to acid-base imbalance. Carbon dioxide is a potential acid; when dissolved in water‚ it becomes carbonic acid. Lungs under the
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1. The given margins of the two products as indicated in the reports submitted by the accounting department for the drums and mountain bike frames should not be used in the decision of which product to make. This is due to the following reasons: a. The product margins are lower than they should be‚ simply due to the fact of the presence of allocated common fixed costs that happen to be irrelevant in making this decision. b. Moreover‚ when the fixed costs are taken out of the equation‚ the profitability
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WATER/BUFFER LECTURES .....SOME NOTES YOU SHOULD HAVE TAKEN IN CLASS \wxNotesInClassBe. 1301124 8 and Things you should have done‚ or tried... = all problems! Gas pressure/concentration: 1 Atm = 760 mm Hg = 10 m H20 = 15 psi (psi is the industrial unit). O2 and man: upper limit/ lower limit Minimum: Without adaptation‚ man can not survive on top of Mt Everest = 8‚000M = 0.3Atm = height of jet liners= 0.06 Atm O2 Man starts being affected at 0.5 ATM air = 18‚000’ Maximum
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Africana Studies 1-1-2011 ‘The Mystic Drum’: Critical Commentary on Gabriel Okara’s Love Lyrics Chukwuma Azuonye University of Massachusetts Boston‚ chukwuma.azuonye@umb.edu Follow this and additional works at: http://scholarworks.umb.edu/africana_faculty_pubs Part of the African Languages and Societies Commons‚ Comparative Literature Commons‚ and the English Language and Literature Commons Recommended Citation Azuonye‚ Chukwuma‚ "‘The Mystic Drum’: Critical Commentary on Gabriel Okara’s
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08 Fall 08 Fall The Modern Drum Set: Driving the Rhythm of Change in America Jeffrey Ringle A synthesis and comparison of the socioeconomic‚ cultural‚ religious‚ and musical effects the invention of the drum kit has had on America since its invention circa 1900. The Modern Drum Set: Driving the Rhythm of Change in America Jeffrey Ringle A synthesis and comparison of the socioeconomic‚ cultural‚ religious‚ and musical effects the invention of the drum kit has had on America since its
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Analysis on many of the benefits of children skipping rope By Danica Jan 10‚ 2012 The reason for loving skipping: children like the bouncing movement‚ not only well-developed and healthy‚ also the intelligence will be improved. The following is the brief analysis on the four benefits of skipping rope. First‚ the more jumping the better cardiopulmonary function. Skipping rope can speed up blood circulation‚ and improve cardio-pulmonary function and respiratory function; accelerate the gastric motility
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Scheduling Basics DDMRP uses buffered “Decoupling Points” to establish independent planned and scheduled horizons. DDMRP creates resupply signals based on the “available stock” status of each buffer. This gives prioritized sequence based on actual need. On-the-floor priority is then determined by the real time buffer status of the decoupling points that the open orders are feeding. When Will we Need More Scheduling Capability? • When the use of better shop floor scheduling will compress lead times and
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