different concentrations of alcohol to extract red and blue dyes‚ and artificial flavoring. Data: Conclusion: Different dyes are shown when separated by different concentrations of isopropyl alcohol. Discussion of Theory: Chromatography‚ resolution and selectivity played major roles in making the experiment work. The experiment demonstrated a common use of chromatography‚ and works to explain a way to separate mixtures. The dyes were successfully separated and analyzed in the cartridge.
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Introduction In this Lab the students dealt with Osmosis‚ movement of water molecule or solvent from a high concentration to a low concentration‚ through selective permeability‚ a protective barrier that provide some particle the ’direct ’ passage in and out of the plasma membrane ( Lacerda L.2011) Allowing some molecules enter and exit the cell membrane‚ to create a balance in concentration inside and outside of the cell‚ by doing so the cell often become hypotonic‚ where the concentration
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This experiment consisted of two parts. First‚ the absorption of each dye was evaluated by diluting (1 mg/mL standard) 2.0 mL of dye to 100 mL. The absorbance of each dye standard was measured and documented. The absorbance of the Gatorade was then measured by diluting the solution and pipetting 5.0 mL into a 25.0 mL volumetric flask. The absorption spectrum of the chosen drink was measured. Next‚ the purple Gatorade extraction procedure was carried out‚ beginning with pipetting 1 mL of 70% isopropanol
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compounds composed of nitrogen atoms bearing alkyl or aromatic compounds. Amines undergo interesting reactions‚ one of which is with the reaction with nitrous acid producing an azo dye. In this study‚ the experiment focused on synthesizing an observing the physical properties of Sudan-1. Sudan-1 is of the most common dyes found in waxes‚ oils and in some food ingredients specifically curry and chilli powder. Furthermore‚ this study aimed to understand the mechanism behind the synthesis of 1-phenylazo-2-naphtol
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chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds’ (Wikipedia‚ 2011). This method is similar to thin layer chromatography‚ but the compounds in the mixture are collected separately. Green dye is used in this experiment that consists of blue and yellow constituents. Materials and Methods: As per SCI105 Chemistry laboratory manual 2012‚ Brooks‚ 2012‚ page 12-19. With the following changes: The mass of recrystallization mixture
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Exocytosis Lab Report Rough Draft Abstract: In Paramecium caudatum‚ trichocysts are 10^3 secretory vesicles that are released from the cytoplasm. We first observed how the presence of Alcian blue dye triggers normal secretion of trichocysts‚ and we then tested four different hypotheses in order to determine how the presence of Ca++ ions in the extracellular and intracellular environment affects the secretion of trichocysts. Our first our hypothesis tested whether extracellular calcium is
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What is American Federalism? According to Dye (2011)‚ "nations are not truly federal unless both national and subnational governments exercise separate and autonomous authority‚ both elect their own officials‚ and both tax their own citizens for the provision of public services" (p.263). American Federalism is just that. In the United States each state has their own government with elected officials‚ to go even further each town within these states also have their own elected officials. These officials
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liter sample is dispensed into well by holding the tip of the micropipette just inside the well so as not to puncture the bottom. 7. We continued inserting the remaining dye samples into each of the remaining wells‚ using the same amounts for each well. For example: Well #2 contains Dye sample #2 and Well #3 contains Dye sample#3 and so on. 8. After we loaded the E-Gel‚ we started the 35-minute electrophoresis run. This was confirmed by a steady green light. 9. The current automatically
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Ashley Spees CHM 261- Batzer Chem Final Write Up- Kool Aid Lab Total Acid This part of the lab requires titration of Kool Aid with an NaOH solution. The reason titrations are used is to determine the concentration of an unknown solution by reacting a strong acid with a strong base. Titrations are hard to accomplish‚ though‚ due to the fact that indicators used to show the endpoints are very sensitive and one drop could make the solution titrate past its endpoint. Blue stock solution: 3 mg/L
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OBJECTIVES: Given a set of activities‚ the students should be able to attain at least 75% proficiency and should be able to: A. describe staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological
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