ALCIAN BLUE RATIONALE FOR USE Alcian blue is a water soluble‚ amphoteric copper phthalocyanine‚ which is most often used as a basic dye. In general‚ alcian blue has an affinity for sulfated and carboxylated groups of acid mucopolysaccharides‚ but the specific group demonstrated is dependent on the dye solution pH. RECOMMENDED FIXATIVES 10% neutral buffered formalin and Bouin’s fixative. AVOID Chromate Fixatives MODE OF ACTION (1‚ 2‚ 3) Alcian blue binds electrostatically with
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antibody. (Labelling phase) Bio-Rad Protein Assay The Bio-Rad Protein Assay‚ based on the method of Bradford‚ is a simple and accurate procedure for determining concentration of solubilised protein. It involves the addition of an acidic dye to protein solution‚ and subsequent measurement at 595 nm with a spectrophotometer or micro plate reader. Comparison to a standard curve provides a relative
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com Liquid Chromatography AP Chemistry Laboratory # 18 Publication No. 10535A Catalog No. AP9093 Introduction In this experiment‚ liquid chromatography is used to separate the substances that are present in grape-flavored Kool-Aid®. First‚ the dyes responsible for the purple color‚ FD&C Blue #1 and Red #40 are separated. Then‚ in a second experiment‚ the other components of Kool-Aid®‚ the flavorings and citric acid‚ are separated as well. Concepts • Resolution • Liquid chromatography • Selectivity
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dyeing of hair is an ancient art. In ancient times‚ the dyes were obtained from plants.[ Some of the most well known are henna (Lawsonia inermis)‚ indigo‚ Cassia obovata‚ senna‚ turmeric and amla. Others include katam‚ black walnut hulls‚ red ochre and leeks.[16] In the 1661 book Eighteen Books of the Secrets of Art & Nature‚ various methods of coloring hair black‚ gold‚ green‚ red‚ yellow‚ and white are explained. The development of synthetic dyes for hair is traced to the 1860s discovery of the reactivity
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Abstract The Bradford method used to determine the protein content of a certain solution (Menguito‚ 2010) and involves the acidic Coomassie Brilliant Blue G-250 as a coloring reagent. [1] The dye is originally pinkish-brown in color when it is in its acidic state. When protein is bound to the dye the color turns blue. In this experiment a standard was used to start the experiment‚ specifically the BSA stock solution. This standard served as the “control” group of the experiment. Due to the
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Methods and Materials In this experiment‚ three dye molecules were used to calculate the solubility of the chemical structures in the relation to Rf . The Carmine dye was used because it contain six –OH bonds. Since‚ six –OH bonds were identified in this molecule the Rf results obtained‚ should differ greatly from the results taken from the other molecules. Alizarin (red) dye was chosen because it has one -OH bond. The third molecule Azure chosen because it does
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(cm) D2(cm) Calculated Rf Component Rf (from table 2) Green Colouring Yellow‚ Blue B: 8.15 Y: 6.55 8.15 B: 1.0 Y: 0.80 B: 1.0 Y: 0.89 Unknown Mixture Red‚ Yellow‚ Blue R: 5.72 Y: 6.18 B: 7.10 7.10 R: 0.81 Y: 0.87 B: 1.0 R: 0.78 Y: 0.89 B: 1.0 Table 4 Dye Red #2 Red#3 Red#4 Yellow#5 Yellow#8 Blue #1 Blue #2 Rf 0.81 0.41 0.62 0.95 0.77 1.0 0.79 Observations The solvent (water) always travelled farther than the solute‚ except in the case of blue‚ which travelled the same amount as the solute. The blue
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exciting solutions happening throughout the life cycle of this pioneering industry. Impacts and Concerns The environmental footprint of creating apparel varies based on the methods used to produce and extract the resources; the process to create‚ dye and finish the materials; the steps to cut‚ sew and assemble the products‚ and the packaging and distribution systems to move and deliver the product. Examples of the environmental impacts of these steps are discussed below: Conventional Cotton Farming
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The U.S Constitution was written in 1787. The Founding Fathers “recognized that government depended on the consent of the governed” (Dye & Gaddie‚ 80). The citizens of the United States give the government permission to have a government. The Founding Fathers are very significant because they provided limits on the power of the majority and safeguarded the rights of the minority. The Bill of Rights also provides safety for the minority and does not allow the majority to exceed their power. The paradox
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the experiment‚ the stationary phase was the filter paper onto which the dye samples were dropped onto while the mobile phase was the solvent mixture containing ethanol and water which carried the sample dyes as the mixture went up the paper at different rates. These rates depend on the solubility and polarity of compounds in solvent. The objectives of this experiment are to compute the retention factor values of 6 sample dyes: methyl
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