PRACTICAL 7 TITLE : DETERMINATION OF ERYTHROSINE CONCENTRATION USING UV- VISIBLE SPECTROPHOTOMETER INTRODUCTION In chemistry‚ spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that spectrophotometry deals with visible light‚ near-ultraviolet‚ and near-infrared‚ but does not cover time-resolved spectroscopic techniques. Spectrophotometry
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example. Because the DNA is invisible‚ the loading buf fer also contains two dy e s : bromophenol blue (a small dye molecule that behaves like a DNA fragment about 600 bases long) and xylene cyanol (a larger dye that acts like a DNA fragment of about 4000 bases). These dyes form lines that give you an idea of how far your DNA has moved. Some loading buffers also have a third dye‚ behaving like a very small DNA molecule (50 bases or so). As the DNA migrates‚ the different fragments will form
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------------------------------------------------- Chemistry It is a synthetic dye produced using aromatic hydrocarbons from petroleum.[1] It can be combined with tartrazine (E102) to produce various shades of green. It is usually a disodium salt. The diammonium salt has CAS number [2650-18-2]. Calcium and potassium salts are also permitted. It can also appear as an aluminium lake. The chemical formation is C37H34N2Na2O9S3. The dye is poorly absorbed from the gastro-intestinal tract and 95% of the ingested dye can be found in the feces. It also
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gram-positive bacteria will retain the crystal violet-iodine complex. Gram-negative bacteria will not retain the color and the decolorizer will wash out the stain. F. What is the purpose of safranin in the Gram’s stain procedure? This is a basic dye that directly stains the gram-negative bacteria that have become decolorized. The gram-positive bacteria are already stained and
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Study guide for Microbiology Chapter 2 Name________________ 1. List and describe the sequence steps routinely used to identify bacteria. These are referred to as the “five I’s” in your text. 2. Define / describe each of the following as they apply to microbiology: a. Culture f. pure culture b. Inoculum g. contaminated culture c. Inoculation h. mixed culture d. Colony 3. Microbiologists employee a number of approached to acquiring a pure culture
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necessary as Lab Paq provided pre-diluted chemicals. Utilizing the 96-well plate‚ 2 pipit drops of each chemical were added to the wells in the following combinations: a) NaHCO3+HCl b) HCl+Bromthymol blue c) NH3+1 drop Bromthymol blue d) HCl+blue dye e) Blue dye+NaOCl followed by HCl f) NaOCl+KI followed by various test foods g) KI+Pb(NO3)2 h) NaOH+phenolphthalein i) HCl + phenolphthalein j) NaOH+AgNO3 k) AgNO3+NH3 l) NH3+CuSO4. Along the way‚ observations were made pertaining to the reactions witnessed
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Particle in a Box Lab March 25‚ 2014 Kassandra Brady‚ Samantha Imler‚ & Michael Montone CHEM 353 Abstract: Introduction: Method I: Absorption Spectra of Conjugated Dyes This method analyzes a series of dyes with alternating double bonds. It is assumed that the π electrons are free to move and ignoring the electro static repulsion among the π electrons themselves. The theoretical model applied in this experiment is the particle in the box. The model states that a single particle moves free
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Hair coloring or lightening is the practice of changing the color of hair. During lightening‚ hair goes through various stages until it reaches the ideal orange/gold color. Lightening time varies‚ depending on natural hair color. The darker the hair‚ the longer the lightening time needed. Medium brown hair on average takes somewhere around 40 minutes to lighten. Dark brown hair takes somewhere around fifty minutes to lighten. Darkest brown hair takes around sixty minutes to lighten. And black hair
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DESIGN 9 2. Nucleic acid purification 9 3. Reverse transcription 9 4. Controls and normalization 9 5. Standard curve evaluation of efficiency‚ sensitivity‚ and reproducibility 9 Real-Time PCR Fluorescence Detection Systems 12 DNA-Binding Dyes 12 Primer-Based Detection Systems 13 PROBE-BASED DETECTION SYSTEMS 14 Hybridization probes (also called FRET probes) 16 MELTING CURVE ANALYSIS 16 Multiplex real-time PCR 18 APPLICATIONS OF REAL TIME PCR 18 GENE EXPRESSION ANALYSIS 18
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(Master budget preparation) Sopchoppy Company manufactures a red industrial dye. The company is preparing its 2000 master budget and has presented you with the following information. 1. The December 31‚ 1999‚ balance sheet for the company is shown below. SOPCHOPPY COMPANY Balance Sheet December 31‚ 1999 Assets Liabilities and Stockholders’ Equity Cash $ 5‚080 Notes Payable $ 25‚000 Accounts Receivable 26‚500 Accounts Payable 2‚148 Raw Materials Inventory
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