have limitations. The Coomassie dye only interacts with certain amino acids such as: arginine‚ histidine‚ lysine‚ tyrosine‚ tryptophan and phenylalanine. However‚ each amino acid has different structure from each other; therefore the Coomassie dye will interact differently with each amino acid. The Coomassie dye molecules are bound to proteins by elctronstatic attraction enhanced by hydrophobic bonding (Tal et al. 1984). Besides the interaction between Coomassie dye and amino acids‚ some compounds
Premium Amino acid Coomassie Protein
the differential dye Wright-Giemsa solution (a mixture of methylene blue‚ eosin Y‚ and Azure B). Compare this to the slides (stained‚ unstained) of your cheek cells you prepared. In your answer‚ consider the effect/purpose of the active reagents in the fixative‚ the permeabilization solution‚ and stains (methylene blue‚ DAPI‚ Texas Red). For example‚ why are the “fix” and “permeabilize” steps needed when using a reagent like Texas-Red®-phalloidin‚ whereas they are not with a dye like methylene blue
Premium Protein Performance Blood
Material not properly desized Material not properly mercerised. Absorbency of the fabric not proper Sticking of insoluble material on the fibres Impurities are not removed properly Uneven heat treatment. 2. Poor machine conditions • Coming out of Dye liquor during dyeing • Defective instruments controlling temperature‚ pressure speed etc. 3. Poor water quality • • • • More Hardness of water Water has metal ions such as iron. PH of water not proper Water having more chlorine 4. Short comings in
Premium Printing Textile
Dairy - It is a common activity in many villages. People feed their buffalos on various kinds of grass and the jowar and bajra that grows during rainy season. Then the milk is sold in nearby villages and towns. It is alos transported to far away towns and cities. A dairy is a business enterprise established for the harvesting of animal milk – mostly from cows or goats ‚ but also from buffalo ‚ sheep ‚ horses or camels – for human consumption. A dairy is typically located on a dedicated dairy farm
Free Milk Indus Valley Civilization India
glow sticks work. But what are the chemicals compounds that react chemically in a light stick. In a typical commercial light stick there is a mixture of hydrogen peroxide solution and a solution containing a phenyl oxalate ester and a fluorescent dye. Here’s the sequence of events when the two solutions are combined: 1. The hydrogen peroxide oxidizes the phenyl oxalate ester‚ resulting in a chemical called phenol and an unstable peroxyacid ester. 2. The unstable peroxyacid ester decomposes
Premium Light Incandescent light bulb Thomas Edison
national chemical engineering school in 1904. In 1909‚ this young chemist founded the company L’Oreal. He had an amazing “entrepreneurial spirit”. Schueller began by selling his own formulated hair dye to the Persian Hairdressers. The next year after that‚ he convinced Paris hair stylist to use his hair dyes for a fashion show‚ with his determination and ambition he succeeded and earned publicity. Another bit of information that we know is that Schueller had a passion for chemistry always trying new
Premium Human physical appearance Hairdressing Hairstyle
plant biology‚ aiding this study are the fundamentals of histology and techniques in histochemistry. This experiment involved exercising free-hand sectioning techniques‚ learning maceration procedures and application of staining techniques. Sudan dye for Lipids and IKI test for starch were applied on section stem‚ root and leaf parts of Tridax procumbens. Stem cross-section of T. procumbens showed distinct ground tissues and fourteen to sixteen vascular bundles organized into a ring. The root
Premium Leaf Plant anatomy Root
What will happen when you put a drop of water on wax paper? I think that it will just sit there and do nothing. First I put a drop of water on the paper Second I watched it to see what it will do Finally I wrote down that it just stayed still My hypothesis is correct What will happen when you move the water around with a toothpick? I think it will go flat and dissolve in the in the wax paper. First I picked up the toothpick Second I moved the water around with the toothpick Finally
Premium Liquid Ethanol Oil
The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
Premium Protein Spectroscopy Electromagnetic radiation
the ability of bacteria cell wall to retaining the crystal violet dye or methylene blue. The cell walls for Gram positive microorganisms have a higher peptidoglycan and lower lipid content than gram negative bacteria. Bacteria cell walls are stained by the crystal violet. Iodine is then added as a mordant to form the crystal violet and iodine complex so that the dye cannot be removed easily. This step is usually called fixing the dye. However‚ the next treatment with a decolorizer‚ which is a mixed
Premium Gram staining Staining Microbiology