observations of the reactions were recorded in Data Table 1. A1) Two drops of NaHCO3 and two drops of HCl. B1) Two drops of HCl and two drops of BTB. C1) Two drops of NH4OH and one drop of BTB. D1) Two drops of HCl and two drops of blue dye. A2) Two drops of blue dye and two drops of NaOCl. One drop of HCl was then added. F) Two drops of KI and two drops of Pb(NO3)2. G) Two drops of NaOH and two drops of phenolphthalein. H) Two drops of HCI and two drops of phenolphthalein. B2) Two drops of NaOH
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Small amounts of household cleaning products 1 Goggle-safety 1 Well-Plate-24 1 Well-Plate-96 1 Pipet‚ Empty Short Stem 1 Aqueous Ammonia‚ 1M -1mL 1 Bromthymol Blue‚ 0.04% - 2 mL in Pipet 1 Copper (II) Sulfate‚ 0.2 M – 2 mL in Pipet 1 FDC Blue Dye #-1.0.1% - 2 mL in Pipet 1 Hydrochloric Acid‚ 1.0M-1 mL 1 Lead (II) Nitrate‚ 0.2 M- 2 mL in Pipet 1 PhenolphthaleinSolution1%1mL 1 Potassium Iodide‚ 0.1 M-2 mL in Pipet 1 Silver Nitrate‚ 0.1 N – 2 mL in white Dropper Bottle 1 Sodium Bicarbonate
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Enzyme Immobilization Methods Covalent Binding: Covalent binding is a conventional method for immobilization; it can be achieved by direct attachment with the enzyme and the material through the covalent linkage [37]. The covalent linkage is strong and stable and the support material of enzymes includes polyacrylamide‚ porous glass‚ agarose and porous silica [38]. Covalent method of immobilization is mainly used when a reaction process does not require enzyme in the product‚ this is the criteria
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electrophoresis: Sensitivity‚ mechanisms‚ and DNA electrostretching ‚ Mutation Research/DNA Repair‚ Pages 167-175‚ Retrieved October-9-2012 3. Siguang Jiang‚ Xiaodong Chen‚ Minghua Liu‚ (2004)‚ The pH stimulated reversible loading and release of a cationic dye in a layer-by-layer assembled DNA/PAH film‚ Journal of Colloid and Interface Science‚ Pages 396-403‚ Retrieved October-9-2012 4. Robert F.Weaver. Molecular Biology. (5th ed.). McGraw Hill International Edition‚ United States. 76-79pp 5. Keren DF‚
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the byproduct of the two is a base. C NH 3 and BTB When two drops of Ammonia and one drop of Bromothymal blue are combined the result remains blue. This means they are base. D HCI and blue dye When Hydrochloric acid and blue dye are combined the result is a base. E Blue dye and NaOCI When Blue dye and Sodium Hypochlorite react are combined the result is green. This means it is a base. F NaOCI and KI When Sodium Hypochlorite and Potassium Iodide are mixed the two combined produced a reddish
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the legs. Activation of either labellar or tarsal gustatory neurons with a sugar solution results in proboscis extension‚ which is a component of feeding behavior Flies have neuron on their legs‚ wings and body that activate whenever sugar is near (Dye 2004). Flies such as fruit flies eat nectar because it is
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identified. For example‚ at 100x‚ a direct stain of yeast returned a cluster of cocci. A stain is a chemical that adheres to structures of the microorganism and in effect dyes the microorganism so the microorganism can be easily seen under a microscope. Stains used in microbiology are either basic (direct) or acidic (indirect). Basic dyes are used for positive or direct staining
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You have learnt many staining techniques through the lab exercises: What dye did you use in simple stain? Crystal Violet What dyes did you used in Gram stain? Crystal violet‚ gram’s iodine‚ and safranin violet. Why did you have to heat up the slide when you did the acid fast stain and the spore stain? To open the spores and allow the dye to enter and stain. Once cooled down the spores closed trapping the dye inside. What color is a “+” in acid fast stain? Which bacteria are “+” for
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buffer provided the correct pH for the gel to run. The solution would be heat until the agarose is completely dissolved. Ethidium bromide solution would be added to the solution after it cooled slightly. Ethidium bromide solution was a fluorescent dye that intercalates between bases of nucleic acids and allows very convenient detection of DNA fragments. It enabled visualization of the fragments within the gel under UV light. The solution would be poured into the gel mold for solidification. The gel
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dish • Incubator • Refrigerator • Bunsen burner • Gas connection • Plastic tubing • Inoculating loop • 12 sterile glass slides • Wax pencil • Igniter • Crystal violet dye • Gram’s iodine • Ethyl alcohol • Safranin dye • Paper towels • Wire rack • Sink • Brightfield compound microscope • Lens paper • Immersion oil • Pen and paper Methods I. Collecting the environmental specimens: 1. Place some
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