Isolation of Recombinant Taq Polymerase for PCR Isolation of Recombinant Escherichia coli IPTG induced Taq polymerase and characterization through polymerase chain reactions‚ Western Blotting and gel electrophoresis * Braeden Cowbrough1‚ Michael Atkins2‚ Christopher Bonner3 From the Faculty of Biochemistry Lab 3006 B Carleton University‚ Ottawa‚ ON K1S 5B6 *Running title: Isolation of Recombinant Taq Polymerase for PCR To whom correspondence should be addressed: Braeden Cowbrough‚ Faculty of Biochemistry
Premium Polymerase chain reaction Molecular biology
metals arranged in order of their reactivity based on displacement reactions‚ reduction of their oxides by hydrogen or carbon‚ the relative ease of decomposition of their compounds and reactions with oxygen‚ dilute acids and water. The higher up a metal is in the reactivity series‚ the more reactive it is. In the experiment conducted‚ the three metals‚ magnesium‚ zinc and copper each had to undergo displacement reactions and reactions with water‚ oxygen and dilute acids‚ to arrange them in the order
Premium Zinc Oxygen Metal
Coupled Reactions Louella Rose E. Tan‚ Frances May L. Coralde Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines Results and Discussion A combustion reaction is a reaction that liberates heat and light. Magnesium is a stable element and reacts violently with evolution of much heat and light. The reason the magnesium burned in carbon dioxide was because it combined with the oxygen in the CO2 leaving elemental carbon behind. CO2(g) C(S) + O2(g)
Premium Adenosine triphosphate Carbon dioxide Oxygen
Redox reactions (Redox terminology‚ galvanic cells‚ standard electrode potentials‚ strength of oxidizing/reducing agents‚ corrosion and protection against it) Oxidation is defined as the loss of electrons and reduction is defined as the gain of electrons. Oxidizing agents‚ such as oxygen are defined as substances which accept electrons; reducing agents are substances which donate electrons. Redox reactions are: a) The reactions of metals with non-metals 2Na+Cl2→2Na+Cl- b) The reactions
Premium Electrochemistry Hydrogen
REACTIONS OF HYDROCARBONS Vanessa P. Manibpel De La Salle University - Dasmariñas ABSTRACT Five substances namely Hexane‚ Eugenol‚ Unknown hydrocarbon 1‚ Unknown hydrocarbon 2‚ and Acetylene gas was used for the selective reactivity of hydrocarbons to functional group tests; Bayer’s test‚ Bromine test light‚ Bromine test dark‚ and Tollen’s test. In Bayer’s test‚ only Acetylene‚ Eugenol and Unknown hydrocarbon 2 reacted positively and the rest retain the purplish color of KMnO4. On the other hand
Premium Hydrocarbon Alkene Functional group
Redox Reactions Reactions involving oxidation and reduction processes are very important in our everyday world. They make batteries work and cause metals to corrode (or help to prevent their corrosion). They enable us to obtain heat by burning fuels--in factories and in our bodies. Many redox reactions are complex. However‚ combustion and synthesis (from elements) are two ordinary examples which require very little description. Just a little more involved are the displacement reactions‚ with
Premium Chlorine Hydrogen Bromine
Properties and Reactions of Hydrocarbons Abstract Introduction Hydrocarbons are organic materials that contain only carbon and hydrogen atoms‚ these molecules can be saturated or unsaturated and acyclic‚ cyclic‚ or aromatic. In this experiment we used a variety of methods to examine the physical and chemical properties of hydrocarbons. The first experiment we tested a variety of solvents to see if they dissolved our alkane (Decalin). One would expect that non-polar solvents will dissolve
Premium Solvent Solubility
BIOL2220 Lab 3: Hill Reaction Introduction In 1937‚ Robert Hill discovered that isolated chloroplasts can generate oxygen when they are illuminated in the presence of a suitable electron acceptor‚ even in the absence of carbon dioxide. This finding was a landmark in the study of photosynthesis because it established that the source of the electrons used in the light reactions is water. It also confirmed that the released oxygen is derived from water instead of carbon dioxide. In chloroplasts
Premium Photosynthesis
Observations: Part I: Reaction between iron and oxygen Appearance of steel wool after 20 minutes From (relatively) shiny metal strips to not-so-shiny strips that are stained with dark‚ reddish-brown stuff. Part II: Decomposition of hydrogen peroxide Appearance of potato and hydrogen peroxide combination: Lots of white‚ foamy bubbles around the potato slices. Day 2 Observations: Part III: Reaction between zinc and acetic acid Appearance of penny in vinegar after
Premium Oxygen Carbon dioxide Sodium bicarbonate
Polymerization reaCTIONS polymers are high-molecular-weight compounds‚ fashioned by the aggregation of many smaller molecules called monomers. Ex. plastics that have so changed society and the natural and synthetic fibers used in clothing are polymers. Classification of polymers Homopolymers - consist of repeated long chains or structures of the same monomer unit. Ex. PVC(polyvinyl chloride) Copolymers - polymers that consist of more than one molecule. Trimers - molecules consisting
Premium