2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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This lab is adapted from the canned denatured pineapple experiment. Instead of investigating the effect of canning on enzyme activity‚ we look for the presence of the substrate. This is a useful teaching lab for all the criteria and requires very few resources. Background Information: Gelatin is made from a protein called collagen which comes from the joints of animals. Gelatin may be dissolved in hot water. As the dissolved gelatin mixture cools‚ the collagen forms into a matrix that
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Introduction Enzymes are protein based structures that help speed up chemical reactions. They help these reactions keep up with the everyday metabolic needs and other like functions of organisms. Enzymes are also considered catalysts‚ due to the lowering in activation energy‚ in which they are not consumed or changed at any point during the reaction. These enzymes have three main protein structures that help keep them formed and intact. Stage 1 of these structures is the primary structure‚ which
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How many will it hold Benchmarks Covered (Learning Objective) SC.8.N.1.1 Define a problem to plan and carry out scientific investigations. SC.8.P.8.2 Differentiate between weight and mass‚ recognizing that weight is the amount of gravitational pull on an object and is distinct from‚ though proportional to‚ mass. Research Question/Problem - Which type of pasta noodle would hold the most pennies if we were to tape them to two stools 16 cm apart‚ dropping pennies into a cup tied around the noodle?
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Jennifer Anatomy and Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat
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Ye Tao BISC220-13155 The Effect of Temperature on the Digestion of Starch by Activity of Enzyme α-Amylase: Observation of Rate of Starch Disappearance through Iodine Test Introduction An enzyme is a type of protein that‚ through its own structure including hydrogen bonds‚ acts like a biological catalyst and is able to accelerate the biochemical reaction rate by lowering the activation energy of the whole process‚ without which cells could hardly practice any physiological functions within human
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time goes on the stronger paramecium will eventually take over‚ and the weaker paramecium will disappear. 2. Explain how you tested your hypothesis. (Give details of how you conducted your experiment. Consider this the “Methods” section of a lab report.) I filled on test tube with the Caudatum paramecium‚ another test tube with Aurelia paramecium‚ and the 3rd test tube contained both parameciums. Each test tube was tested every 2 days by using wet mount slides. Day 0: Tube 1‚ 2‚ and 3 remained
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Celestial Geography I Emily Richarz PH090-04 Purpose: The purpose of this lab was to introduce us to star charts and how to use them to find stars‚ constellations and other bodies in the sky. Procedure: The instructor gave a brief but thorough lecture and demonstration on how to go about finding the answers on the worksheet. We then filled out the worksheet that cover finding stars and constellations using right ascension and declination in general and on specific dates‚ where constellations
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The purpose of this lab is to successfully infiltrate E. coli bacterial cells with a pARA-R plasmid that is antibiotic resistant and has the rfp gene‚ or red fluorescent protein. This can be verified if the E. coli obtains the characteristics of the plasmid when it enters. To start‚ three Petri plates containing agar are needed. On each plate there is a control group and a treatment group; the treatment group being the one with the plasmid. Before the plasmid is put with the E. coli‚ first the bacteria
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Lab Lesson 9: Geologic Time (p.203) due end of week 3 Return to Assessment List Part 1 of 1 - 100.0/ 100.0 Points Question 1 of 31 3.0/ 3.0 Points Questions 1 through 3 are based on the Lab Exercise‚ Step 2. Record your answer to Lab Exercise‚ Step 2‚ Question 11. How long ago was the igneous rock in rock unit B formed? A. 352 mya B. 380 mya C. 704 mya D. 1408 mya Answer Key: A Question 2 of 31 3.0/ 3.0 Points
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