Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3 #
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producing a form of stilbene (Figure 1). This reaction favored a crude Z-Stilbene crystal product over its E counterpart. When Z-Stilbene underwent photoisomerization with iodine for 1 hour it reconfigured almost exclusively into its more stable counterpart E-Stilbene. The reaction produced very low yield of 6.3% due to the nature of the reaction and the speed at which iodine reacts. The purity of E-Stilbene could have been increased by allowing the reaction to perform longer and to use a faster reactant
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METHODS In this experiment‚ plasmid lux and a control plasmid (pUC18) will be introduced into E. coli by transformation. There are four basic steps to the procedure; Preparation of competent cells (These steps should be performed by the instructor.) 1. Place a vial of CaCl2 solution and the tube of E. coli in the ice bath. 2. Using a sterile pipet‚ transfer 590µL CaCl2 solution to the tube containing 50µL of the bacteria. 3. Tap the vial with the tip of your index finger to mix the solution. 4. Incubate
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Introduction to Management Science‚ 10e (Taylor) Chapter 9 Multicriteria Decision Making 1) The different objectives in a goal programming problem are referred to as goals. Answer: TRUE Diff: 1 Page Ref: 400 Main Heading: Goal Programming Key words: goal programming 2) All goal constraints are inequalities that include deviational variables. Answer: FALSE Diff: 1 Page Ref: 400 Main Heading: Goal Programming Key words: goal constraints 3) A negative deviational variable is the amount by
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structure of the enzyme is mainly dependent on the active site and variable groups. Extreme temperatures or extreme pHs can alter the structure of an enzyme. Enzymes function to lower the activation energy to break the bonds. They achieve this by putting stress and pressure on the bonds or creating a microenvironment for the substrate. Enzymes are regulated by inhibitors or activators and can be inhibited by the products of the reaction‚ called feedback inhibition. Enzymes are catalytic proteins;
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r Virtual Lab: Enzyme Controlled Reactions Instructions 1. Go to the following web-link in order to open the Virtual Lab: http://www.mhhe.com/biosci/genbio/virtual_labs_2K8/labs/BL_02/index.html 2. Open the Virtual Lab: Enzyme Controlled Reactions 3. The virtual lab simulation will be on the right side of the screen‚ and the “Question” column will be on the left side of the screen. 4. Click the monitor in the lab simulation to watch a video about enzyme action. 5. Click the “Information”
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Science Study Notes An electrically uncharged object is neutral. Conduction is not a method of charging an object. If electric charged stay in one spot‚ they are said to be static. In charging by induction‚ when a negatively charged rod is brought near a metal leaf electroscope the electrons in the electroscope move away from the rod. A statement that is not part of the Law of Electric Charges is that neutral objects are attracted to uncharged objects. The purpose of a GFCI is to protect people from
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doctor: He founded the Laboratoire de recherches medico-legales de Montreal‚ the first laboratory in North America and only the third in the world. 8. It is important to get a measurement and diagram of the scene so it can be recreated on a computer. 9. A crime scene technician would use a camera to photograph the scene‚ a UV light to find clues that arent visible to the naked eye‚make diagrams and take measurements‚ they would use a brush and fingerprint powder to make any fingerprints more visible
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Enzyme kinetics (catalase/kmno4) Enzyme catalysis Farah Mohamed galal 22-3014 t09 Introduction: E + S → ES → E + P Enzymes are proteins which act as a catalyst in biochemical reactions(affect the rate of achemical reaction). The substrate binds to the active site of the enzyme. Any deformation of the active site will affect the activity of the enzyme‚ these are some ways that enzyme action may be affected because of them: 1- Salt concentration: If it is close to zero or very high
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Physical Science lab #5 Objective The goal of this experiment is to develop a theory‚ which allows us to understand the motion of a marble. Materials Wooden roller coaster‚ marble‚ ruler‚ timer‚ clamp‚ physics stand. Theory In this experiment‚ we are finding the Conservation of Energy. Energy is neither created nor destroyed. Energy is summed up into two different properties: Potential energy and Kinetic energy. The law of Energy states that: Total Energy = Potential
Free Energy Potential energy Conservation of energy