Title: E. Coli Transformation with a Plasmid DNA Containing the GFP Gene Introduction: Bacterial transformation is the process of bacteria taking in and expressing exogenous DNA. This has led to many other discoveries. In order for bacterial transformation to occur the bacteria must be in a certain physical state to be able to take in DNA. This is called competency and it allows the cell membrane to be permeable so DNA can pass through. Currently researchers are studying the transformation
Premium DNA Plasmid Gene expression
Rate of Enzyme Activity Justin Hunter G. Kim September 16‚ 2011 September 26‚ 2011 Abstract Catalase is a common enzyme found in nearly all living organisms that is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less reactive oxygen and water molecules. Catalase is a protein that is most commonly found in the liver. The purpose of this experiment was to determine the effect that changes in temperature and pH have on the function of the enzyme catalase
Premium
Science 10 Examination Booklet 2011/12 Released Exam for T/S Form A DO NOT OPEN ANY EXAMINATION MATERIALS UNTIL INSTRUCTED TO DO SO. Examination Instructions 1. On your Answer Sheet‚ fill in the bubble (Form A‚ B‚ C‚ D‚ E‚ F‚ G or H) that corresponds to the letter on this Examination Booklet. 2. Use a pencil to fill in bubbles when answering questions on your Answer Sheet. 3. When the examination begins‚ remove the data pages located in the centre of this booklet. 4. Read the Examination
Premium Mathematics Science Question
Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
Premium DNA Gene Molecular biology
and peanuts [F4-Chapter 6] Q2: To determine the concentration of the solution which will maintain the mass of plant tissues. [F4-Chapter 3] 2006 Q1: To study the effect of concentration of albumen suspension on the rate of reaction of pepsin enzyme. [F4-Chapter 4] Q2: To determine the concentration of sucrose which is isotonic to the cell sap of mango. [F4-Chapter 3] 2007 Q1: To study the effect of light intensity on the rate of photosynthesis of a Hydrilla sp. [F4-Chapter 6] Q2: To study
Premium Enzyme Glucose Starch
SC131: Human Anatomy and Physiology II Unit 9 Student Name: __________________________ Unit 9 Lab Worksheet: Endocrine Anatomy Directions: Go to Real Anatomy and select Endocrine. Find the appropriate images to use as references and use your mouse to roll over the images and click on the highlighted structures. You can use the search engine to find additional images. If you need help navigating Real Anatomy‚ click on Take a Tour or click the question mark for the Help menu. 1. Identify the highlighted
Free Thyroid Endocrine system Organ
New Building-Optical Networks The new structure has been given a grant to make its network state of the art by making it a fiber optic network. The topics of discussion that will be mentioned in this paper are the hardware that will be necessary for the inside and outside of the building and how we would expand the fiber to an adjacent building if it is built. Another topic to be discussed will be describing the safety procedures that will be following during the installation. INSTALLATION HARDWARE
Premium Management Marketing OSI model
Lab 6. Respiratory System Mechanics-14 Name ______ Purpose: This lab explores the mechanics and factors which affect the process of pulmonary ventilation‚ the exchange of gases between the outside of body and the lungs. Materials: Physio Ex CD 9.0 Procedure: Complete the activities on Respiratory System Mechanics in the lab manual. Read the lab and answer the questions‚ some of which do not come from the lab manual‚ and submit.
Premium Asthma Lung Respiratory physiology
Virtual Lab: Enzyme Controlled Reactions Worksheet 1. Which of the following does NOT apply to an enzyme: Inorganic 2. When an enzyme catalyzes a reaction: Substrate(s) bind in the active site 3. Which of the following would interfere most with the ability of an enzyme to catalyze a reaction? Reduced concentration of substrate available 4. Feedback mechanisms regulate the rate of enzyme activity‚ effectively “turning off” an enzyme in a
Premium Enzyme PH Chemical reaction
Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
Premium Plasmid Bacteria Molecular biology