WESTERN BLOTTING (Written Report) I. PRINCIPLE Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight. In Western blotting‚ proteins are electrophoresed into a gel‚ as the proteins migrate through the gel they are separated based upon size and charge. Characteristically‚ smaller proteins migrate through the gel faster than larger proteins. Sufficiently separated proteins in an SDS-PAGE can be transferred to a
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Western Blotting is used to Identify a Subunit of Myosin Light Chain in the Proteins of All the Different Fish. Chae Yee Kent State University: BSCI 30140 Lab Abstract Western Blotting can be used to detect the Myosin actin light chain in different species of fish and is used to distinguish from different species based on variation‚ commonality‚ or evolutionary divergence. First‚ proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according
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Eastern Airlines Facing Bankruptcy In 1986‚ Eastern Airlines was in desperate trouble. The fourth quarter of 1985 had shown a $67.4 million loss‚ and financially experts had told Frank Borman‚ president and chief executive officer‚ that the airline had three choices: 1) a 20 percent pay cut for all union and noncontract employees. 2) Filing for Chapter 11 (bankruptcy) or 3) Selling the airline. On February 23‚ 1986‚ Eastern’s board of directors met to decide the
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EASTERN EQUINE ENCEPLALITIS Before researching for this paper‚ I had never heard of this disease. Eastern equine encephalitis is a disease that affects mostly horses. It however can affect humans‚ leading to very serious complications. According to Center of disease control and prevention (CDC)‚ (2004-2013) EEE is most prevalent in areas that are swampy and wet. An environment that will encourage breeding of mosquitoes. Mosquitoes are the primary means that humans get this serious
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Western Civilizations HMS-301 Section A Project Topic: Futurism Prepared By: Bassel Paoli (12110518) Instructor: Dr. Karim Tannous Introduction Futurism Futurism is an artistic and social movement that began in Italy Milan in 1909 by the Italian poet Filippo Tommaso Marinetti. The movement launched under the name Futurist Manifesto‚ written by Filippo‚ was published in the Italian newspaper Gazzetta dell ’Emilia in Bologna on 5 February 1909‚ and later on in French on 20
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Politics and the State in Western Europe ca 1450-1521 Due to the ineffective leadership‚ the nation states of Frances‚ England and Spain utilized aggressive methods to rebuild their governments in the fifteenth century. This was accomplished though the revival of the monarchy and the unification of nations. The French leaders led their people and nation states to success. Charles VII‚ a sovereign viewed as weak and frail‚ proved himself a powerful leader. Charles (reigned from
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Common Elements of Eastern Religion REL/133 January 07‚ 2012 Common Elements of Eastern Religious Many religions of the world have eight elements in common. The elements are a belief system‚ community‚ central myths‚ ritual‚ ethics‚ characteristic emotional experiences‚ material expression‚ and sacredness. These elements help shape religions and the people who believe in them. In this paper I discuss how these elements are similar or how they differ in each of a few of indigenous religions
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The political ideology of communism sought to end capitalism and establish equality throughout a population. While good in theory‚ it created conflicts that lasted for decades. First conceived in the Soviet Union‚ there was an expectation that communism would be a revolutionary tide that would sweep across all of Europe. After WWII‚ the Soviet Union still dominated with communist ideals in the countries of Czechoslovakia‚ Hungary and Poland. After the Berlin Wall that separated Germany into East
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want to be professionally. Sure‚ attending NYU has taught me a lot and pushed me academically‚ but I’d like to be able to power down once in a while‚ or at least get out of the ‘study’ setting I’ve been stuck in for nearly two years. I feel that Western would be a better fit to help me do that‚ and allow my focus to shift more to my future after my education and regaining a life outside of schoolwork. Academics have always been important and a
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Modern European Weaponry: Shaping Changes in Warfare The human being has always found a way to inflict harm in the name of defense or simple destruction‚ for survival‚ or for the annihilation of the enemy. All within the realm of warfare‚ we have found a way to make sticks and stones break bones‚ and everything between simple technologies to the advent of gunpowder‚ has changed civilization and the way we live. Throughout this essay we will look at the simple truths that come from an evolution
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