BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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I. Preparation A. Choosing 1. You should choose the fruits that you like. a. For example‚ if you desire to gain more different fruits enzymes or make the glass bottle more colorful‚ you can choose four or even more kind of fruits. There is no limitation. But‚ you must include one fruit‚ lemon. It helps to kill the bacteria of the drink. B. Composing 1. The ratio of components should be 3‚ 1. It means 3 parts of fruits‚ 1 part of sugar. You can follow this ratio to determine the amount of fruit
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TASK 4: Explain the structure of the ileum and the ultrastructure of the epithelium in relation to its secretory and absorptive functions. (a) Explain the characteristics of enzymes. (b) Explain the lock and key theory of enzyme action. (c) Locate and explain the role of important digestive enzymes. TASK 5: Enzymes are involved in anabolic and catabolic reactions: a) Define the terms anabolic and catabolic reactions b) Give 3 examples of anabolic and 3 examples of catabolic reactions
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Sterling Hayden AP Biology Mrs. Proehl 24 November 2014 I. Title Effect of Differing pH‚ Temperature‚ and Enzyme Concentration on Catalase Reaction Rate II. Introduction Enzymes are used to increase the rate of specific reactions in the body. Catalase‚ a specific enzyme‚ speeds the breakdown of hydrogen peroxide‚ a toxic chemical produced by cells in the body‚ into water and oxygen (Cain and others‚ 2010). The oxygen can be observed as bubbles coming from the reaction site. Catalase is found in many living tissues of organisms
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Effects of Temperature and pH on Enzyme Function Chelsie Mesa Section 0479 Robin Cotter Introduction The purpose of this experiment is to identify three unknown enzymes. This is done by using different temperature and pH to affect the function of the enzyme‚ which ultimately‚ will affect how much maltose is produced. Enzymes are proteins that help catalyze chemical reactions. In enzymatic reactions‚ the molecules at the beginning of the process are called substrates‚ and the enzyme
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The effect of increasing substrate concentration on rate of an enzyme reaction. Enzymes are biological catalysts that lower a reactions activation energy making possible many of the reactions needed for life to exist. Enzymes have a high specificity which have been explained by many theories such as Fischer’s lock and key. Currently the most widely accepted theory is the induced fit hypothesis proposed by Koshland in 1958. This hypothesis solves some of the problems with the Lock and key theory
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The Effect of Starch on the Activity of Amylase with pH Variable Lab Report‚ Fall 2011 East Tennessee State University Department of Biological Sciences By: Shelby Brackett Date Performed: October 10‚ 2011 Lab Instructor: Joseph Kusi Biology 1111‚ Section 018 Abstract Enzymes are very important in chemical reactions. They are used to speed up the reaction taking place. They act by binding to a specific substrate and form an enzyme-substrate complex that may put stress on chemical bonds
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Kenneth Hampton | | |Restriction Enzymes: | |A study in Reactions and Mapping | | | |November 7‚ 2008 | ABSTRACT This experiment will study the
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Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also‚ they can speed up Metabolic reactions without being changed or used up. During a reaction‚ an enzyme molecule combines temporarily with the substrate. When the reaction is complete‚ the enzyme molecules returns to its original dorm
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Enzymes Ashley Njeru Mrs. Booth Tuesday‚ March 19th 2013 SBI 4U INTRODUCTION: The cellular activity of all organisms is controlled through the use of enzymes. An enzyme is a special type of biological molecule‚ usually a protein‚ which speeds up a chemical reaction; most are soluble in water or a dilute salt solution. There are about 4000 different enzymes in a typical living cell. If even one of these enzymes is missing or
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