Lambda DNA with restriction enzymes. Aim: The objectives of this experiment are: Become more familiar with using micropipettes. Use restriction enzymes to cut DNA at specific sites. Use Ligase to rejoin some of the cut/separated DNA fragments. Learn to separate DNA using electrophoresis. Introduction: Restriction enzymes are proteins which cut dsDNA at specific regions depending on the enzyme used‚ determined by the nucleotide sequence of the DNA‚ i.e. each enzyme recognises specific nucleic
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An investigation into the effects of sugar concentration on yeast activity Introduction: Yeasts are eukaryotic micro organisms belonging to the kingdom fungi. Yeasts live on sugars and produce ethanol and carbon dioxide as by-products. [James Mallory‚ 1984]When Yeasts are given water and sucrose they convert the sucrose into glucose then convert the glucose into carbon dioxide and ethanol following the following reaction: C₆H₁₂O₆ ( 2(C₂H₅OH + CO₂ [Brady Burkhart‚ Terrell Grayson and Eric Kimler
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do not protect the environment well‚ the nature will have bad effect on us; sometimes we may lose our property‚ even lives. For example‚ the snow and ice disasters occurred during the last winter. No doubt‚ forbidding supermarkets and shops from handing out free carriers would be inconvenient for the consumers. They have to carry the bags by themselves when they go shopping. Or they pay for the plastic bags. They cannot use the free plastic bags as the rubbish bags at home any more. They may feel
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reaction involves enzymes. So to understand how bleach works‚ we first understand how enzymes work. Enzymes Enzymes are the most important thing in our lives‚ it makes the world go ‘round! Enzymes are proteins that acts as catalysts and help combine or break down hydrogen peroxide. Imagine a pen with ink inside it‚ when you write‚ ink comes out‚ right? You can draw‚ you can write‚ then when you finish and get your pen off of the paper‚ the ink stops coming out. Enzymes are like that‚ they
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The aim of this experiment was to investigate the effects of salt type and concentrations on the rate of heating and the boiling point. Then from this data make a conclusion which salt type and concentration is the most energy efficient at heating water to its boiling point. It was hypothesised that highest concentration of MgCl2 was going to be the most energy efficient at heating the solution to its boiling point. This is because MgCl2 has the lowest specific heat capacity thus will use the least
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Potato Enzyme Lab INTRODUCTION An enzyme is a protein that speeds up or slows down a specific chemical reaction in an organism. A good rule of thumb is to remember that enzyme names end in “-ase”. This will help in identifying enzymes in further readings. Generally enzymes are catalysts. Hydrogen peroxide is a toxic chemical that is produced in many organisms during metabolism. Organisms must get rid of this toxin to survive. One reaction turns the hydrogen peroxide into water and oxygen. The
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organisation is accurate and efficient and delivered in a timely and cost effective manner and that the human resource data is managed professionally. The behaviours describe in detail how a human resources professional needs to carry out their activities. Each behaviour is described at four bands of professional competence. There are eight behaviours which I have listed below:- 1. Curious – Is future focused‚ inquisitive and open minded; seeks out evolving and innovative ways to add value
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Melting Point and Boiling Point of Organic Compounds Bongo‚ Sayre‚ J1 1Student‚ Organic Chemistry 1 Laboratory / B11‚ School of Chemical Engineering‚ Chemistry and Biotechnology‚ Mapúa Institute of Technology ABSTRACT The melting point of a substance is the temperature at which the material changes from a solid to a liquid state while the boiling point is the temperature at which it changes from liquid to solid. In this experiment‚ the main objectives were to determine the effects of the following
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Restriction Enzymes Restriction enzymes (also known as restriction endonucleases) are a group of bacterial enzymes which cut double-stranded DNA (dsDNA) into smaller fragments at specific points. They are a defence mechanism used by bacteria to cleave the DNA of invading viruses‚ thereby restricting their expression. The exploitation of restriction enzymes ability to cut large pieces of DNA into smaller fragments (called restriction fragments) and the highly specific way in which they do this
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785-532-0708 • jkoleen@ksu.edu Course Description Welcome to First Year Seminar in K-State Culture! Instead of listening passively to lectures‚ you will directly experience what a university is by attending the varied cultural and intellectual activities that occur at K-State. In this class‚ you will learn about the very broad range of experiences you can have in college‚ you will develop critical thinking skills‚ and hopefully you will come to understand how these experiences can make you a more
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