BIOL 140 Lab—The Chemical Composition of Cells Name ____________________________ I) Introduction All cells contain four major types of macromolecules: carbohydrates‚ lipids‚ nucleic acids‚ and proteins. In today’s lab‚ we will be studying three of the four-proteins‚ carbohydrates and lipids. Various chemical tests can be used to detect the presence of each of these molecules. Most of the tests involve a color change visible to the eye. If a color change is observed‚ the test is considered positive
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Simple Diffusion 1. The following refer to Activity 1: Simulating Dialysis (Simple Diffusion). Which solute(s) were able to pass through the 20 MWCO membrane? According to your results‚ which solute had the highest molecular weight? ______________________________________ Which solute displayed the highest rate of diffusion through the 200 MWCO membrane? __________________________ Using the data from Chart 1‚ explain the relationship between the rate of diffusion and the size of the solute. Facilitated
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MOVEMENT ACROSS MEMBRANES Intracellular fluid Extracellular fluid Pond water Blood plasma K+ A- ClNa+ Cl+ K+ Na+ Cl- Na+ Na+ Gill epithelial cell Intestinal epithelial cells Cl- Cell membrane Anionic proteins (a) Ion concentration inside a single animal cell (b) Ion concentration across gill epithelium of a freshwater fish Blood capillary Intestinal lumen Glucose from meal Cross section of small intestine (c) Glucose transport across intestinal epithelium into
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Testing Cell Transportation Across a Membrane Introduction Cells have the amazing ability to transport certain molecules in or out of their membrane. Some require no energy to do so (passive transport) while others require energy to be processed through (active transport). There is also the transportation of water across a membrane‚ which has its own term of osmosis. Too much of something can be taken in‚ or too little enters. This especially happens to plants‚ who require water (and sun) to live
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the effect of different concentrations of ethanol on the permeability of beetroot cell membranes. Prediction: By exposing a membrane to a solvent‚ ethanol‚ it will increase its permeability. So the higher the concentration of the solvent‚ the more permeable the membrane will be. But if the concentration of the ethanol is beyond a certain limit‚ it may break down the cell membrane to the point where there is in fact no overall effect as the ethanol would disturb the lipids in the membrane.
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Exercise 1: Cell Transport Mechanisms and Permeability: Activity 4: Simulating Filtration Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Filtration is a process that You correctly answered: c. is passive. 2. Filtration is dependent upon a You correctly answered: b. hydrostatic pressure gradient. 3. The filtrate You correctly answered: d. All of these answers are correct. 4. An important place that filtration takes place in the body is in You correctly
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Core Practical 2.8 Investigating why the colour leaks out of beetroots soaked in alcohol Planning Aim: - to investigate the effects of different concentrations of alcohol on the membrane structure of a beetroot a) Decide what you think will be the effect of alcohol on beetroot cell surface membranes and how this will affect their permeability. Write down your idea as a hypothesis that you can test‚ and support your idea with biological knowledge. Hypothesis “The greater the concentration
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hypothesize about membrane traffic in lab‚ explain the differences between the solutions hypertonic‚ hypotonic‚ and isotonic and how they respond using the understanding of the cell membrane structure‚ types of transport mechanisms such as active‚ passive‚ diffusion‚ osmosis‚ and explain the movement of particles moving across the cell membrane. In this lab was divided into two parts. The first part was varying the concentration and the second part was varying the temperature. All cells are controlled
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Effects of Tonicity on Cell Membrane Abstract The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells‚ potato strips and three unknown solutions (A‚ B‚ C). First three slides were prepared containing RBC’s and unknown solutions A‚ B and C. A control slide was prepared only using RBC’s. After observing each slide under the microscope it was determined that unknown solution A was hypertonic because the RBC appeared to have shrunk. The
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Content Frame PhysioEx 9.0 PhysioEx 9.0 is easy-to-use laboratory simulation software that consists of 12 exercises containing 63 physiology lab activities. Exercise 1: Cell Transport Mechanisms and PermeabilityDownload the Exercise 1 Review Sheet:PDF Download Exercise 1 Review Sheet PDFRTF Download Exercise 1 Review Sheet RTF Exercise 2: Skeletal Muscle PhysiologyDownload the Exercise 2 Review Sheet:PDF Download Exercise 2 Review Sheet PDFRTF Download Exercise 2 Review Sheet RTF
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