Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this
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appear. Results and Conclusions 1. Contained in the following chart: • Concentrations are calculated by multiplying the concentrations by the amount of liters of that solution divided by the total volume. • -∆S2O8-2 is calculated by dividing the concentration of the S2O3-2 by the number of moles required‚ as seen in the chemical equation (2 moles) • ∆t was determined during the lab
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The activity of an Enzyme is affected by its environmental conditions and changing these factors can alter the overall rate of the reaction. Reaction rates are influenced by external factors such as pH‚ temperature and salt concentration. Different enzymes have different optimum temperatures in which they are most efficient and different pH levels which is ideal for their formation of enzyme-substrate complexes. As the temperature increases‚ there is more movement of molecules and more random collisions
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Enzyme Catalysis Lab Problem: Before the lab‚ one should understand: • The general functions and activities of enzymes; • The relationship between the structure and function of enzymes • The concept of initial reaction rates of enzymes; • How the concept of free energy relates to enzyme activity; • That change in temperature‚ pH‚ enzyme concentration‚ and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and • Catalyst‚ catalysis‚ and catalase
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Investigating the effect of changing substrate concentration on the activity of the enzyme catalase The aim of this experiment is to examine how the concentration of a substrate (hydrogen peroxide) affects the rate of reaction of an enzyme catalyse (found in liver cells) Research Question: how does changing the concentration of the substrate affect the rate of reaction of the enzyme catalyse? Hypothesis: As the concentration of the substrate increases‚ so does the rate of reaction until the reaction
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Lab activity 1: Effect of temperature‚ concentration‚ and pressure on equilibrium Introduction Our ongoing discussion has been on systems at dynamic equilibrium: for a reversible reaction‚ the rate of the forward reaction is equal to the rate of the reverse reaction. What happens if equilibrium is disturbed? In this lab activity‚ we are going to examine the effect of changing reaction conditions on the position of equilibrium. Part I: Effect of temperature We will consider the equilibrium
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Introduction I am going to try and measure the rate of respiration in yeast and find out what factors affect the rate of respiration. I am going to change the concentration of the glucose solution and I’m going to measure the volume of gas produced during respiration in cm³. Yeast contains enzymes. Enzymes speed up a chemical reaction – they’re biological catalysts. Yeast can respire both aerobically and anaerobic; the anaerobic respiration of yeast is known as fermentation. The equation for
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Wan Ching Man 5C(21) Effects of temperature on the activity of lipase Aim: To investigate the effects of temperature on the activity of lipase enzyme on milk which contain fats or lipids. Introduction: Enzyme is a kind of biological catalyst that made up of protein. It can speed up the metabolic reactions on various kinds of substances. Like lipase can break down lipid into glycerol or fatty acids in milk. Since enzyme is made up of protein which easily affected by varies temperature. This
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Ah Seung Chong Molecular Biology CTW: Enzyme Kinetic Dr. Cruz 07/22/2010 Enzyme kinetics Introduction Enzymes are biological catalysts or assistants‚ without enzyme many of important processes of life could not happen. Most of enzymes are proteins that help speed up chemical reactions by lowering amount of activation energy needed for the reaction1. Enzymes are usually highly selective‚ only bind to specific substrate and convert it to product at a particular rate1. The rate of the reaction
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flask using a volumetric pipette. The volumetric flask was then filled to the mark with distilled water. The flask was covered with Parafilm and inverted to mix. The next part of the experiment was to determine the Vitamin C in a solution of known concentration. Observations of the Vitamin C sample and the amount found on the label was recorded. A tared piece of weighing paper was used to weigh 0.27 g of Vitamin C. The Vitamin C was then transferred to a clean 50 mL volumetric flask. The flask was filled
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