pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
Premium Enzyme Starch Chemistry
The Effect of pH on Enzyme Activity A piece of Solanum tuberosum (potato) was removed and mixed with distilled water in a blender. The resulting solution was filtered through multiple layers of cheese cloth to filter out the liquid by eliminating any large pieces in the solution. The solution created was catechol. Five different solutions were prepared as blanks with each test tube containing 6.0mL of a different pH (pH 4‚ pH6‚ pH7‚ pH8‚ pH10) of phosphate buffer‚ 1.0mL of the enzyme and 1.0mL of
Premium Enzyme Chemistry Catalysis
Enzymes are a type of protein. They are catalysts meaning they speed up the rate of the reaction. Enzymes activity depends on the concentration of the substrate‚ temperature and the pH. The more concentrated the substrate is the more reactive the enzyme is. The optimal pH for an enzyme is 7.5 and the optimal temperature for an enzyme is 53 Celsius. Extremes in the temperature and the pH of an enzyme can denature therefore destroy it. The enzyme that is in this experiment is Amylase. Amylase is found
Premium Enzyme Chemical reaction Catalysis
Aim: The rate at which a reaction occurs‚ or takes place‚ is affected by various factors‚ such as the temperature at which the experiment is conducted in‚ the pressure which is given‚ the surface area which is exposed‚ use of catalysts‚ and the concentration. In this investigation‚ I would be investigating the affect of surface area‚ or particle size‚ in the rate at which a reaction occurs. Background Information: The more finely divided the solid is‚ the faster the reaction happens. A powdered
Premium Chemical reaction Hydrogen Magnesium
Enzymes are biological molecules (proteins) that act as a catalyst and help complex reactions occur everywhere in life‚ for example a piece of steak that is being digested into energy. Molecules found at the beginning of the process are called substrates‚ and these enzymes exchange them into differing molecules known as products. Nearly all-metabolic processes in a cell need enzymes in order to function at rates that are fast enough to sustain existence. Those who are lactose intolerant are simply
Premium Enzyme Chemical reaction Metabolism
"Do different concentrations of solutions determine the mass of a potato?" Introduction: The way to get the full results of this lab was through the process of osmosis. Osmosis is the movement of water across a membrane into a more concentrated solution to reach an equilibrium. When regarding cells osmosis has three different terms that are used to describe their concentration. The first of these words is isotonic. Cells in an isotonic solution show that the water has no net movement and the amount
Premium Concentration Solution
15th Influence of pH on Enzyme Activities How does the pH of solution influence the speed of enzyme activities? Enzymes are protein catalysts and chemical that speed up chemical reaction without being involved in the reaction. They are involved in the human digestive system to help people digest food more efficient. Food are substrates to be break down and absorb through the digestive system in the body. Substrate is a molecule that bond with the active site of enzyme to transfer in to one or
Premium Enzyme Chemical reaction Metabolism
Determination of Rate and Order of a Reaction Results This experiment used a spectrometer to find the wavelength with maximum absorbance in a green food coloring solution. For this particular solution the wavelength was 629.7 nm. The system was then calibrated to that and was set to measure the food coloring and bleach solution. The measured visible light absorbance of the mixed solution was collected over a time of 200 seconds and eight points were then selected and placed into the Absorbance
Premium Rate equation
Reaction Order and Rate Law Name Data‚ Calculations‚ and Questions A. Calculate the initial and final concentrations as needed to complete Tables 1 and 2. Data Table 1: Varying the Concentration of 1.0 M HCl | | | | |Concentrations | | |# Drops |# Drops |# Drops |Initial
Premium Chemical reaction Rate equation Reaction rate
experimental group had a huge impact when the diluted concentration of caffeine was put in compared to the control group that contained no concentration of caffeine. The hypothesis was supported because a significant decrease of the total number of cells when caffeine solution was added to the experimental group was clearly shown. An addition of small amounts of caffeine does inhibit cell proliferation and affect cell viability during the lab experimentation one more test was conducted because the
Premium Experiment Science Hypothesis