Reminder: All post labs need to be 1- typed (not handwritten) ‚ 2- original (not copied from a classmate)‚ 3- answered using complete statements and 4- turned in at the beginning of the lab. Post-lab questions for Topic 5 – Enzymes Name: Date: Group: T W R Formation and Detection of Benzoquinone Table 1. Formation and Detection of Benzoquinone: Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate
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Effects of Temperature and Cofactors on Enzymatic Reactions “I pledge that no unauthorized assistance has been given or received in the completion of this work. Experiments described were performed by me and/or my lab group and this write-up is entirely my own creative work.” X________________________________________ Introduction Enzymes are protein molecules that speed up the rate of reactions by reducing the activation energy of a reaction. They act as catalysts in reactions‚ increasing
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Determining Order from Rates of Reactions AP Chemistry Purpose The focus of this experiment is to recognize that when aqueous solutions of potassium iodate ion (KIO3-) and bisulfite ion (HSO3-) are mixed‚ a series of reactions will occur‚ and the final reaction is signaled by the appearance of a dark blue color. My partners and I investigate how the concentration of the reactants affects the rate of reaction. The purpose of this lab is to find the order
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Effect of pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st‚ 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body‚ enzymes
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Chemistry 1225 Lab Write-Up #13 Abstract Electrolysis is a method of using a direct electric current to drive an otherwise non-spontaneous chemical reaction. During electrolysis‚ hydrogen atoms (H2) are reduced at the cathode whereas the oxygen atoms (O2) are oxidized at the anode. There were three different solutions used in the experiment in order to have a better understanding of electrolysis reactions. From each solution‚ reaction equations were produced. The reactions could be observed from
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CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40‚000 different enzymes in human cells‚ each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times‚ allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner‚ and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration
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Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life’s processes would not come so easily. Enzymes are basically proteins‚ which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down
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The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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I. Title. Restriction Enzyme Mapping of pBR322 Using Agarose Gel Electrophoresis. II. Authors. Author: Partner: Section: Thursday‚ 1:10 pm Date of Experiment: October 25‚ 2012 III. Introduction. Restriction enzymes (or restriction endonucleases)‚ originally isolated from Haemophilus influenzae in 1970‚ are enzymes within a cell that cleave foreign DNA within a specific and predictable nucleotide sequence (known as a restriction site) regardless of the source of such DNA. Such restriction
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Defining the problem and selecting variables In this lab‚ we will be studying the difference of heart rate between boys and girls. There are many things that can increase heart rate‚ such as exercise‚ drinking coffee‚ taking certain pills‚ drinking an energy drink‚ feeling a strong emotion‚ etc. I have chosen to use exercise as a means of increasing the heart rate of my subjects‚ more precisely‚ 20 sit-ups. There are many reasons why your heart rate increases when you exercise‚ the most important one
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