In this investigation I will be measuring the effects of temperature on the membrane permeability of beetroot. I will be measuring the amount of anthocyanin that will diffuse out of the beetroot. The way in which I will measure the anthocyanin is to check the light absorbency of the solution using a colorimeter. The higher the reading on colorimeter the more anthocyanin present in the solution To find out the permeability of the beetroot membrane I will firstly cut out cylinders of beetroot using
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Plan:- Independent Variable- Temperature is what I will be changing in the experiment. I want to find out what effect temperature has on the permeability of a cell membrane. Controlling other Variables- I will use measures to ensure that everything stays the same in my test to gain the best and most reliable results possible - Same volume of water that I put the beetroot into after heating. - The beetroot I use will be the same size each time‚ I will ensure this using a cork borer. - The beetroot
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substances across a semipermeable membrane to gain a better understanding of how cells move materials across cell membranes. The experiment was designed to exhibit Brownian Motion; the random movement and tendency of particles of a substance to move from an area of greater concentration to lower concentration via diffusion until equilibrium has been attained. In addition to observing the passive process of diffusion‚ the experiment demonstrated how a semipermeable membrane will only allow certain substances
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Planning AIM Beetroot cells contain a red pigment‚ which is stored in the cell vacuole and a vacuole membrane to prevent this leaking out of the cell surrounds it. The outer of the cell is also surrounded by a membrane‚ which again helps contain the pigment inside the cell. In this experiment I aim to find out the relationship between the leakage of red pigment from a beetroot cell and the surface area. To do this successfully I will need to alter the surface area of the beetroot cells accurately and then
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Aim: To investigate how effect of Detergent Concentration (cont.) has on Membrane permeability of Beetroot cells. Hypothesis: I predict that as detergent concentration increases‚ the solution will become less clear‚ plus mass increases. The increases in mass will indicate that the water potential of the Beetroot cell is lower than that of the surrounding sucrose solution. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than
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Purpose: Examine the role of the cell membrane in the cell by disrupting its function using temperature (Biology 107 Laboratory Manual 2014). This will improve the general understanding of optimal growing temperatures and the breakdown of the cell membrane Procedure: Betacyanin solution of a known concentration was diluted to create a dilution series‚ then placed in a spectrophotometer set to 525 nm. The absorbance of the dilution was used to create a standard curve for betacyanin. Discs of living
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Investigating the Effect of Temperature on the Permeability of Membranes The permeability of membranes can be altered in several ways. From previous preliminary work I know that reagents‚ for example detergents and alcohol alter the arrangement of the phospholipids in the membrane allowing substances to leak out of the cell. Temperature also affects the membrane. As membranes relay on protein molecules to allow molecules to enter and leave the cell through facilitated diffusion the temperature must
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Implementation My aim for this experiment was to find the effect of temperature on the membrane permeability of beetroot. Since I was unfamiliar with this experiment‚ I first conducted a preliminary test. I considered my preliminary test as my trial for this experiment. My Hypothesis for this experiment was to found out the amount of pigment loss of beetroot when exposed to varying temperatures. Betalain pigment found in beetroot My method for this experiment is:- * Use a cork borer to cut about
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ORGANIC MATTER AND INORGANIC MATTE Organic matter is that which is living or has been alive‚ e.g. wood or coal. Inorganic matter is that which is not living and never has been alive‚ e.g. water or iron. Inorganic substances can be built up into organic compounds by living organisms. All living matter is made up of small units called cells. Some organisms‚ such as bacteria‚ consist of one single cell; others‚ like human beings‚ consist of many hundreds of millions of cells‚ all functioning together
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Introduction To determine the effects of stressful experimental treatments on living membranes we are going to examine how fresh beets roots react when they are exposed to different temperatures. Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell 133). They organize specific chemicals and reactions into specific compartments within the cell. Generally‚ cell membranes consist of phospholipids bilayer
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