We found that‚ in acidic pH environment‚ the reaction rate of starch being broken down by alpha amylase is less than that of the reaction rate at a neutral and slightly basic pH environment. This finding partially supports our hypothesis. The spectrophotometer readings in our experiment measured the absorbance of 3-amino-5-nitrosalicylic acid‚ a colored molecule formed after dinitrosalicylic acid (DNSA) has reacted with the products of the enzymatic reaction or the simple sugars. Therefore‚ the absorbance
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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The Affects of pH on Peroxidase Enzyme Claire Bell Biology 1130 Jazmen Myers Section 36 Abstract Peroxidase is a turnip enzyme; it is used in the oxidation of hydrogen peroxide to lower activation energy‚ speeding up the reaction. The activity of peroxidase is highly dependent on its environment and most importantly the pH level. Peroxidase has been the focus of many recent studies and is believed to possibly reduce swelling among other things. We conducted an experiment testing
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Effects of soil ph variation on radish plants growth Background information: the soil ph can have 2 different impacts on the growth of plant roots. First and most important is how it affects the concentration of nutrients present in the soil itself. This variable varies depending on the ph tendency‚ in particular‚ nutrients like phosphorous‚ potassium ‚sulphur‚ calcium and magnesium tend to drastically decrease in more acidic ph conditions (<6.0)‚ on the other hand iron‚ manganese‚ boron
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Lab : Iodine-starch Clock Reaction Pre-lab: Before the lab was conducted‚ the concentration of the Iodate ions to be in the mixture made by dissolving specific volumes of solution A with a constant concentration and water was calculated using the dissolution formula: C1V1 = C2V2 Sample Calculation 1: Concentration of the Iodate ions: For mixture 1: C2 = C1V1/ V2 = (0.020mol/L) x (0.003L)/(0.01L) = 0.006mol/L The same calculations were used in the calculating of the
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pH and Living Systems I. Purpose: To observe the effects of pH change on an organic molecule. II. Materials: pH paper Droppers Ammonia Beakers (50ml) Paper towels water Glass stirring rods lemon juice forceps III. Procedure: Part 1: Initial pH testing 1) First use the wide range pH paper to test the pH of the liquids given. 2) When you test with the wide range paper first (which reads pH from 0-13) be sure
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materials. The core problem of these starch based plastics is their hydrophilic character and the fact that they tend to become brittle with ageing. Unmodified starch is too much hydrophilic to be used as food packaging material. Chemical modification must be carried out in order to make them hydrophobic. These modifications are grafting‚ alkylation-especially esterification including acetylation. The purpose of the project is to prepare acetylated starches/starch esters of different Degree of Substitution
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pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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digestive power of pepsin is greatest at the acidity of normal gastric juice (pH 1.5–2.5). In the intestine the gastric acids are neutralized (pH 7)‚ and pepsin is no longer effective. "Pepsin (biochemistry)." Encyclopedia Britannica Online. Encyclopedia Britannica‚ n.d. Web. 10 Dec. 2012. Research Question: Does changing the pH of pepsin affect the ability to break down the protein (mass of jello). Variables: independent- Ph level dependent- The mass of the jello
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Name: _____________________ Date: _____________ Student Exploration: pH Analysis Vocabulary: acid‚ acidic‚ alkaline‚ base‚ indicator‚ neutral‚ pH Prior Knowledge Questions (Do these BEFORE using the Gizmo.) 1. Acids are substances that produce hydrogen ions (H+) when dissolved in water. Lemon juice is an example of an acid. A. What does lemon juice taste like? __Sour.______________________________ B. What does it feel like if lemon juice gets in your eye? ___It
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