incubated at 37℃ for overnight. 40mL of a 0.8% agarose gel is prepared after the preparation of the samples was done. Agarose and 1X TAE electrophoresis buffer were added into an Erlenmeyer flask. The 1X TAE electrophoresis buffer provided the correct pH for the gel to run. The solution would be heat until the agarose is completely dissolved. Ethidium bromide solution would be added to the solution after it cooled slightly. Ethidium bromide solution was a fluorescent dye that intercalates between bases
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Books Books are the best friend a person can have. For they are the kind of friends that are never disloyal. A person is never going to feel alone or bored in the presence of books. So‚ books can be read out of passion as a hobby or to gain knowledge and information. For that‚ some books are to be tasted‚ others to be swallowed; and some few to be chewed and digested. There are certain types of books which can only be tasted. To taste a book is just to have some sensations out
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I. Introduction The purpose of this experiment is to determine the pH values of acids‚ bases‚ and buffers of distilled water and 10.0 buffer using measured concentrations of Sodium hydroxide (NaOH) and/or Hydrochloric acid (HCl). Acid is a compound typically having a bitter taste and capable of nullifying alkalis and releases hydrogen ion when added to a solution‚ or containing an atom that can accept a pair of electrons from a base (McKinley‚ Dean O’Loughlin‚ & Stouter Bidle‚ 2016). Bases are water-soluble
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The print “A Voluptuary Under the Horrors of Digestion” by James Gillray portrays Prince George overindulging himself. The photograph “Man shooting up leg” taken by Larry Clark portrays a man shooting up a drug through his leg. Elements within both of these works create a greater theme of addiction. Gillray’s depiction of Prince George sitting tranquilly in a regal chair‚ wearing a blackened shoulder to knee jacket that is on top of a whitened body suit metaphorically being inflated like a balloon
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Temperature and pH will both effect and change the active site of an enzyme. Denaturing‚ the changing of the shape of an enzyme‚ will occur if a big enough change in temperature and pH to go away from that enzyme’s optimal conditions. Each enzyme has optimal conditions which is the best temperature and pH for that enzyme to function. When temperature and pH move away from those conditions the enzyme could slow down its rate of activity
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Billy Kong Chem&163 Lab Report 1 Analysis of Acids‚ Bases‚ pH and Salt Purpose: Explore various methods for determining the characteristics of acid/base solution. The goal of this experiment include the investigation of various acid/base indicators‚ calculating the change of pH with concentration‚ change of pH due to the addition of salt and different between strong and weak acids/bases. Safety and Disposal: Use extreme caution when handling concentrated acids and bases. Dispose of all
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Reaction of Enzyme Amylase Research Question: How will changing the percentage of sodium chloride concentration affect the rate of reaction of enzyme amylase‚ measured using the absorbance of starch and iodine with a spectrophotometer. Introduction: Amylase is an enzyme that is involved in the human digestive process. Found in both the human pancreas and the human saliva‚ amylase breaks down starch into sugar so that large molecules can be easily digested1. Like all enzymes‚ amylase must be kept
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and bacterial amylase‚ as well as the optimal temperature needed for the enzyme to correlate with the bacteria and fungi. The enzyme’s break down within the starch was observed through different temperatures and time periods. The Starch was placed in both the fungal and bacterial amylase where they were then placed on spot plates. Through the iodine test‚ it was concluded whether the breakdown of starch occurred or not. The experiment
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Background An indicator is a halochromic chemical compound that is added to a solution to determine its pH. It is a chemical detector for hydronium ions. The indicator changes colour to show the pH of the solution. Some indicates are not very precise and only tell us whether the solution is acidic or basic. When the indicator is added to the solution‚ they bind to hydrogen or hydroxide ions. The different electron configurations of the bound indicator cause the indicator ’s colour to change.
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William B. Heard Rigid Body Mechanics William B. Heard Rigid Body Mechanics Mathematics‚ Physics and Applications WILEY-VCH Verlag GmbH & Co. KGaA The Author William B. Heard Alexandria‚ VA USA For a Solutions Manual‚ lecturers should contact the editorial department at physics@wiley-vch.de‚ stating their affiliation and the course in which they wish to use the book All books published by Wiley-VCH are carefully produced. Nevertheless‚ authors‚ editors‚ and publisher do not warrant the information
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