Finding the Optimal pH of the Enzyme Peroxidase with the Aid of a Spectrophotometer ABSTRACT The peroxidase enzyme was partially purified‚ and the enzyme activity was calculated with the use of a spectrophotometer‚ demonstrating the effect of pH on peroxidase activity. The objective of this exercise was to determine the ideal pH for peroxidase activity. The data concluded that the optimum pH for peroxidase activity is 5‚ and the “standardized” enzyme volume is 2.8mL
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To investigate the effect of temperature on an enzyme controlled reaction Introduction and planning For the investigation of enzymes‚ I am going to conduct an experiment to see how temperature can affect the rate of reaction of enzymes by testing it with starch. The enzyme that we are going to use is called amylase. We are going to test this enzyme with starch. By mixing amylase and starch solutions together under different temperature conditions‚ we can record the rate of reaction by taking a
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FINC225 Horizontal Analysis of Limited Brand Company Colorado Technical University January 28‚ 2013 Lucille Roxanne Pizzolo-Klein Dr. Ken Nwoke Horizontal Analysis Horizontal analysis is a comparison of ratios and line items from a company to see and forecast how the business is doing and where improvements can be made. It shows overall where problems may lie or where corrections need to be or have been implemented. I did my horizontal analysis on The Limited Brand Company and my results
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INVESTIGATING THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase‚ which is used as a biological catalyst to break down starch‚ which cannot pass through the gut wall due to the size of the molecules‚ into smaller ones. Amylase is a carbohydrase‚ which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific
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PH WANTS ACCESS TO SABAH STANDOFF VICTIMS MANILA‚ Philippines - Philippine diplomats have been sent to Sabah to assist Filipinos affected by thedeadly standoff between Malaysian police and supporters of the Sulu Sultanate‚ the Department of Foreign Affairs (DFA) said on Sunday‚ March 3. “We would like to see how we could work with local authorities in further assisting our nationals affected by the situation‚” DFA Undersecretary for Special and Ocean Concerns Jose Brillantes explained in a statement
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Name: Karen Brockert Date: 01/05/2015 Instructor’s Name: Professor Jackson Assignment: SCI203 Phase 1 Lab Report Title: Human Impacts on the Sustainability of Groundwater Part I: Using the time progression of industrialization and human development‚ fill in the data table below to help you write up your lab report Time Period Impact to Forest Ground water levels plenty Saltwater Intrusion Farming Industrial development Populati on 1800s enormous none minute none limited 1900s Down
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Describe how the uses of plant fibres and starch may contribute to sustainability How can plant fibres be used? First of all the plant fibres have to be extracted from the plant itself. The process of extraction often used is called retting. Retting involves using water or micro-organisms to dissolve or rot away the cellular tissues surrounding the bast-fibre bundles. Afterwards the fibres are obtained; usually they extract very long sclerenchyma cells and xylem tissue because of their good
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can predict that the tube with the highest concentration of yeast will produce the most carbon dioxide and will have the fastest rate of alcoholic fermentation. Results Two separate experiments were conducted to determine the effect of yeast concentration and pH level on the rate of fermentation. The first experiment involved the placement of different concentrations of yeast‚ glucose‚ and distilled water in four different tubes. Pipette pumps were placed on each tube to allow the solution
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What pH Level Causes a Plant to Grow Best Alexis Chavez‚ Heather Nutt‚ Nguyen Phan Introduction We knew that water is normally stated as the best form of liquid to make sure plants grow as best as they can‚ but we wanted to know if causing the water to be more basic or more acidic would affect how the plant grew. Our hypothesis was that if the plant is treated with a pH of 12 then treated with another pH. Materials The materials used were three identical plants‚ that each had 4 sections that were
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transmission at pH 2. At pH 9 the Trypsin has 39% light transmission. Between pH 2 and pH 9 the percentage of light transmission decreases at a steady rate‚ until it reaches pH 8 where there is a steep increase from 30% to 39% as the enzyme has reached its optimum pH at 8. During the experiment in the boiling tube this pH had the deepest red colour as the most protein gelatine was broken up. This meant that in the colorimeter when testing to see what the light transmission‚ this pH let the least light
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