enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To test the optimal pH‚ the starch and a buffer were combined at a specific pH level and
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are amylases? Amylases are enzymes that break down starch or glycogen. Amylases are produced by a variety of living organisms‚ ranging from bacteria to plants and humans. Bacteria and fungi secrete amylases to the outside of their cells to carry out extracellular digestion. When they have broken down the insoluble starch‚ the soluble end products such as (glucose or maltose) are absorbed into their cells. Amylases are classified based on how they break down starch molecules i. α-amylase (alpha-amylase)
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What amount of amylase is the best to produce a better reaction with starch? A 1% solution of amylase‚ made from 0.15g of amylase added to 15ml of water‚ a 2% solution of amylase made of 0.3g of amylase added to 15ml of water‚ and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created‚ and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test
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AN INSIGHT INTO THE WORLD OF CHANDIGARH The City Beautiful Chandigarh is a city and union territory in India that serves as the capital of two states‚ Punjab and Haryana . It is also referred to as The City Beautiful due to its beautiful surroundings‚ central grid of gardens‚ each dedicated to different species of flora. The city of Chandigarh was the first planned city in India and is known internationally for its architecture and urban design. The city has projects designed by architects
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Experiment 3: Soil pH measurement Introduction Aims The aims of the experiment were to determine the pH of a variety of soils which included sedentary‚ 3:2‚ sand‚ organic matter with the means of a pH meter at various soil water ratios and with or without calcium chloride. Method and Materials Samples of sedentary soil‚ 3:2 soil‚ sand‚ organic matter and compost‚ 100ml vials(x12)‚ bottle of distilled water‚ analytical balance‚ pH meter‚ 2 buffer solution of known pH‚ 0.25M calcium chloride
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Determination of pH Almira‚ Faerie Carleen Lucile L. Gallardo‚ Charlotte O. Group #6‚ Chemistry 18.1‚ MHEG1‚ Ma’am Arlou Angeles September 23‚ 2013 I. Abstract The acidity of the four unknown solutions were determined with the use of colorimetry using McIlvaine’s buffer solutions varying in proportion of its constituents (disodium phosphate and citric acid). These buffer solutions were subjected to the addition of corresponding pH indicators and the variation of colors depending on its pH level was
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can reach a new pH level by interacting with soil. If water has a pH that is too high or too low‚ it can be life threatening to aquatic species and can corrode metal structures. After finding this out‚ I decided to test if water could reach an extreme pH just by interacting with soil around it. The question for this experiment therefore became the following. Will interaction with soil change the pH of water? I hypothesized that if water interacts with soil for three days‚ then the pH of the water will
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Introduction: The purpose of this lab was to check the time needed for salivary amylase and phosphorylase to produce a negative result and how different concentrations affected those times. Enzymes are biological catalysts that can cause a specific chemical change in any part of the body (Walsh‚ 2002). Many of the reactions that take place within a cell would normally take place at temperatures substantially higher than those present inside a cell (Alberts et al.‚ 2010). Because of this‚ these chemical
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Title: pH and buffer solutions Aim This experiment was carried out to determine the role of buffer solution and the factor which affect the buffer capacity. Besides‚ this experiment was carried out to investigate the solubility of protein casein over a range of pH concentration. This experiment also was carried out to determine the isoelectric point of the casein and the effect of the isoelectric point toward the casein solution. Methods Verification of the Henderson-Hasselbalch equation
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SLII The effect of three different pH levels (4‚ 7‚ 10) on the rate of germination of spanish beans Research Question: What is the effect of three different pH levels (4‚ 7‚ 10) on the rate of germination of spanish beans? Independent Variable: The different pH levels in the water would be the independent variable in this lab. Three different pH levels were tested‚ 4‚ 7 and 10‚ both 4 and 10 were chosen because they are 3 pH levels away from 7 which would be the regular pH level of water
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