Determination of the Effect of pH on Amylase Activity Grace Chung Abstract: Amylase is an important enzyme in the human body as it allows for the consumption of starch by breaking the polysaccharide down into maltose units. All enzymes‚ including amylase‚ function best at a certain optimal pH. Therefore‚ in this experiment‚ the effect of different pHs on the reaction rate of amylase is studied. It was hypothesized that the amylase-starch reaction would proceed fastest at a pH closest to that of
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The Effects of Heat on the pH of the Vegetable and Their Pigment PURPOSE The purpose of this experiment is to see how pH levels of certain vegetables change while being cooked in four different mediums (frying‚ boiling‚ steaming‚ roasting)‚ and how the varying pH levels change the coloring of the vegetables. We will analyze how the different method of cooking/heating changes the levels of pH. BACKGROUND INFORMATION This experiment is based around the different pigments in vegetables‚ of which
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Enzymatic Activity of Salivary Amylase Abstract: Salivary amylase is an enzyme that can digest starch molecules and break them down to sugar molecules. In this experiment‚ the enzymatic activity and specificity of salivary amylase was examined depending on the changes in pH and temperature. In the first part of the experiment‚ the effect of temperature was determined‚ using constant temperature bath (4‚ room temp‚ 37‚ 50‚ 60‚ and 70°C). Having the room temp and 50°C as the highest and 37°C as infinite
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Summary Expectations: Example Summary (15 total points for every summary) Student Name Partner(s): None Chem 253—TA: TA Name Lab 1: Effect of pH on a Food Preservative (we’ll used this as an example) Date of Experiment Purpose (1 point) Should be a very brief statement such as: To determine the effect of pH on the food preservative Sodium Benzoate. Theory (2 points) In this section you should briefly summarize what the textbook says in regards to the history and uses of
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The role of salivary amylase in the digestion of starches remains controversial. In the absence of pancreatic amylase‚ the key enzyme for starch digestion‚ salivary amylase may well represent a potential compensatory alternate pathway for the digestion of amylose‚ amylopectin‚ and glycogen. Clinically significant depression of pancreatic amylase occurs in chronic pancreatitis‚ pancreatic resection‚ pancreatic neoplasm‚ cystic fibrosis‚ and other causes of pancreatic insufficiency. Quantitation
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Self-design Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme
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The effect of Lead ions on amylase activity Aim What is the effect of Lead ions on the enzyme Amylase. And does it have an inhibitory effect‚ which causes the substrate‚ in this case starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible‚ which is put on the amylase. Method Apparatus and substances required Test tube holder 2% starch solution 6 boiling tubes labelled 1 to 6 1% lead nitrate solution 6 test tubes labelled A to E‚
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MEASURING pH Date of Lab: 02/09/13 Date Report submitted: 02/26/13 Purpose of the experiment By doing this experiment‚ we were trying to learn how to measure a pH with a pH meter to determine whether a solution is weakly‚ moderately or strongly acidic or basic; but also to compare the different data founded to see how the addition of certain liquid to a solution may affect the pH of the initial solution. Materials We used: * Tap water‚ distilled water‚ Soda and milk as the main
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A pH meter is an electronic instrument used to measure the pH (acidity or alkalinity) of a liquid (though special probes are sometimes used to measure the pH of semi-solid substances). A typical pH meter consists of a special measuring probe (a glass electrode) connected to an electronic meter that measures and displays the pH reading. The probe The pH probe measures pH as the activity of hydrogen ions surrounding a thin-walled glass bulb at its tip. The probe produces a small voltage (about 0
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1 Lab Report Title: Measuring pH Levels Instructions: Enter the Virtual Lab‚ and conduct the experiments provided before going out into the virtual field for additional research. Please type your answers on this form. When your lab report is complete‚ submit it to the Submitted Assignments area of the Virtual Classroom. Part I: Answer the following questions while in the Phase 1 lab environment. Section 1: You will be testing 4 known solutions for pH levels using a standard wide-range
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