pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
Premium Enzyme Starch Chemistry
Investigate the effect of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction
Premium Temperature Energy Thermodynamics
Research Question: Effects of pH on amylase activity Introduction: Amylase is an enzyme that is in human’s saliva as well as the pancreas. Enzymes are biological catalysts that speed up a chemical reaction. They break down complex molecules into simple ones. In this case‚ amylase converts starches (complex molecule) into simple sugars. That is why foods like potatoes for example‚ may taste sweet to us‚ because they contain starch. The optimum pH for pancreatic amylase is the pH of 7. In
Premium Enzyme PH Buffer solution
tested the effects of temperature on fungal amylase and bacterial amylase (Aspergillus oryzae and Bascillus Licheniformis). We used 4 different temperatures in Celsius 0‚ 23‚ 58‚ and 89 for both fungal and amylase. For 10 minutes‚ every 2 minutes we would use 3 drops of each amylase and mix it with iodine to observe the presence of starch at each temperature. We conducted this experiment for both bacterial and fungal amylase. Results were reached based on the color of the iodine and amylase mixture
Premium Enzyme Starch Amylase
such as fructose. The enzyme helps a chemical reaction take place quickly so that the reaction happens properly. In order for that to happen the enzymes process by the lock and key model‚ the lock is the substrate and the enzyme is the key. The active sites are specific to a certain substrate of a molecule‚ so the enzymes only have one job to do. The shape of an enzyme is not changed or consumed during these reactions. However‚ without enzymes the reactions would take too long and would not breakdown
Premium Adenosine triphosphate Cellular respiration Glycolysis
Effect of Substrate Choice On Growth Of Mealworms Abstract: The experiment is designed to show which substrate A or B will better nourish a mealworm. A mealworm was placed in each substrate for a period of seven days and the change in mass from Day 1 to Day 7 was compared. The data was compiled and statistically analyzed. The experiment failed to reject the null hypotheses that there would be no difference in the two substrates regarding meal worm growth
Premium Statistical hypothesis testing Null hypothesis
Investigating the effect of pH on amylase activity This practical allows you to: * discover how pH affects the rate of an enzyme controlled reaction * evaluate the experimental procedure Procedure SAFETY: Follow your teacher’s instructions for handling the solutions. Wear eye protection when handling the iodine solution. Investigation * Place single drops of iodine solution in rows on the tile. * Label a test tube with the pH to be tested. * Use the syringe to place
Premium Enzyme PH Buffer solution
collisions between the enzymes and substrates molecules and therefore more reactions. pH is a factor because the different types of enzymes work best in different pH environments‚ a change in pH interferes with the shape of the enzymes active site (where it bonds and reacts with substrates) and therefore does not fit the shape of the substrate as well so the enzyme is unable to work on the substrate. Also changing the concentration of enzyme and substrate concentrations will affect the number of collisions
Free Enzyme Digestion
The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
Premium Enzyme Amylase Starch
Abstract This lab was focused on determining the optimal temperature of the enzyme amylase responsible for catabolizing starch polymers and to see how different temperatures affected the rate as well as how effectively the enzyme worked. To proceed with the experiment the group set up four different test tubes for each‚ bacteria and fungal amylase‚ and labeled them accordingly with different temperatures as well as different solutions . Then the spot plates were placed on the time and temperature
Premium Enzyme Nutrition Chemical reaction