The effect of Lead ions on amylase activity Aim What is the effect of Lead ions on the enzyme Amylase. And does it have an inhibitory effect‚ which causes the substrate‚ in this case starch to be blocked from the reaction process in the enzyme catalyst. Also is the effect reversible or irreversible‚ which is put on the amylase. Method Apparatus and substances required Test tube holder 2% starch solution 6 boiling tubes labelled 1 to 6 1% lead nitrate solution 6 test tubes labelled A to E‚
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Experiment 2: Starch Hydrolysis by Amylase Theoretical Background Polymers of carbohydrates are called polysaccharides‚ and make up some of the most important naturally occurring compounds [1]. They have thousands of monosaccharide units linked to each other by oxygen bridges. They include starch‚ glycogen‚ and cellulose‚ all three of which yield only glucose when completely hydrolyzed [2]. A B Figure 1. Starch (amylose) (A) and cellulose (B) Starch
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Suzuki‚ Laurent Santos‚ Val Justin D.M. Tongco* College of Science‚ University of Santo Tomas‚ Manila Philippines Abstract Salivary amylase‚ found in humans‚ is enzyme that catalyzes the hydrolysis of starch into simpler compounds. Its enzymatic activity is affected by several factors‚ such as temperature and pH. The rates of enzymatic activity of salivary amylase in different temperatures and pH were measured and resulted to be very near 50 C and 7 respectively. However‚ due to some errors that
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known as the active site. The nature and arrangement of amino acids in the active site make it specific for only one type of substrate. Enzymes catalyze an unfathomable number of reactions by using a combination of only six basic mechanisms: (1) acid-base catalysis; (2) covalent catalysis; (3) metal ion catalysis; (4) electrostatic catalysis; (5) proximity and orientation effects; and (6) preferential binding of the transition state complex. Independent of the mechanistic characteristics taken to generate
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Chou‚ P. (2007). The effects of background music on the reading performance of taiwanese esl students. New York: Self Published. Retrieved from http://books.google.com/books?id=zVz_Lv9OnN0C&pg=PA33&lpg=PA33&dq=effects of background music on concentration&source=bl&ots=xRZ4pvia4r&sig=lzJhKQmZfw7WXV-WD1hyJhT9I4A&hl=en&sa=X&ei=I3otUeKHBo_o8gSBooHYAw&ved=0CH8Q6AEwCQ * Hallman‚ Price‚ Katsarou‚ S. J. G. (2002‚ November 2).The effects of background music on
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Analyzing the effects of varying hydrogen peroxide (H2O2) concentrations on the volume of its drop Jonah Jemina IB Chemistry SL 11 Mr. Eastwood Analyzing the effects of varying hydrogen peroxide (H2O2) concentrations on the volume of its drop Jonah Jemina Introduction: Hydrogen peroxide is a clear and colourless substance that has a strong oxidizing characteristic. From the books‚ “Hydrogen Peroxide: Medical Miracle”[1995] by Douglass and “Applications of hydrogen
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OBJECTIVE: The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different
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different sucrose concentrations then the tube with the lowest concentration of sucrose will expand the potato the most because the water will move into the potato to even out the concentration levels of the sucrose/ water ratio inside and outside of the potato was supported by the data. The data shows that the potato submerged in the lowest concentration of the sucrose water solution had the greatest percent change in mass at 15.2% for 0% concentration. Overall‚ the higher the concentration of the solution
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I. Research Question What are the effects of different sucrose concentrations on potato stripes which have been submerged in a range of solute sucrose concentrations. II. Background Information Osmosis is one of the four methods of moving particles across membranes along with simple diffusion‚ facilitated diffusion‚ and active transport. Water is able to move in and out of most cells freely. Sometimes the number of water molecules moving in and out is the same and there is no net movement‚ but at
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Abstract The following experiment details the effect of different concentrations of catalase on the production of oxygen and water through the breakdown of Hydrogen Peroxide. In this experiment paper disc where coated in varying concentrations of catalase‚ 0‚ 25‚ 50 75 and 100%. The time taken for the disc to float between two markers on the side of a glass was then recorded. This experiment demonstrates that the higher the concentration of enzyme used the greater the production of oxygen on the
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