Lab #6: pH Effects and Enzyme Concentration on Catecholase Activity Abstract: This experiment examined the effect on how the changes in pH balance of potato juice affected the enzyme activity‚ structural stability and solubility. Introduction: Enzymes are macromolecules that allow chemical reactions to occur. They function as biological catalysts. A catalyst is a substance that is involved in‚ but not changed by or consumed in‚ a chemical reaction. The amount of energy required for a reaction
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INTRODUCTION Enzymes are biological catalysts that speed up chemical reactions‚ without being used up or changed. Catalase is a globular protein molecule that is found in all living cells. A globular protein is a protein with its molecules curled up into a ’ball’ shape. All enzymes have an active site. This is where another molecule(s) can bind with the enzyme. This molecule is known as the substrate. When the substrate binds with the enzyme‚ a product is produced. Enzymes are specific to their
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To investigate the effect of temperature on an enzyme controlled reaction Introduction and planning For the investigation of enzymes‚ I am going to conduct an experiment to see how temperature can affect the rate of reaction of enzymes by testing it with starch. The enzyme that we are going to use is called amylase. We are going to test this enzyme with starch. By mixing amylase and starch solutions together under different temperature conditions‚ we can record the rate of reaction by taking a
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Date: 14th-15th October Title: The effect of the concentration of hydrogen peroxide on catalase Aim: to examine how the concentration of the hydrogen peroxide affects the rate of reaction Hypothesis: The substrate concentration will determine the rate of reaction. The reaction will increase as the concentration increases‚ but from a certain concentration‚ the rate will maintain the same rate Materials: 3% hydrogen peroxide 6% hydrogen peroxide catalase round metal basin measuring cylinder
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Enzyme Activators and Inhibitors Lucia House AP Biology‚ Block 4 Mr. Trice October 18‚ 2012 Introduction: Metabolism is the totality of all of an organism’s chemical reactions. Chemical reactions occur due to enzymes‚ a substance which acts as a catalyst in driving chemical reactions in order to produce a desired product (Campbell and Reece‚ 2002). A catalyst is usually a protein; however‚ some catalytic molecules counter this generalization. A discovery made in the early nineteen- nineties
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Abstract The following experiment details the effect of different concentrations of catalase on the production of oxygen and water through the breakdown of Hydrogen Peroxide. In this experiment paper disc where coated in varying concentrations of catalase‚ 0‚ 25‚ 50 75 and 100%. The time taken for the disc to float between two markers on the side of a glass was then recorded. This experiment demonstrates that the higher the concentration of enzyme used the greater the production of oxygen on the
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EXPERIMENT TO SHOW THE EFFECT OF DIFFERENT CONCENTRATION OF GLUCOSE ON POTATO STRIPS INTRODUCTION: Molecules of liquid and gas are constantly in motion‚ they move randomly in all directions and bounce around in all directions and bounce around and into each other. As they move‚ they tend to spread out moving from areas with many molecules to areas with fewer molecules . This process of spreading out is called Diffusion‚ for example smell of cooking travelling around the house from the kitchen
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generation of secondary messengers that go on to regulate other physiological processes‚ as well as being responsible for the activation of some ion channels and enzymes and assisting in trafficking of proteins to the cell membrane (5‚ 8). Why is PIP2 then so important in hippocampal cells? PIP2 is a substrate for hydrolysis by the enzyme phospholipase C (PLC)‚ whereby the products of this interaction are the secondary messengers‚ diacyl Glycerol (DAG) and inositol trisphosphate (IP3) (6‚ 8) that
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Title : THE EFFECT OF PH ON THE ACTIVITY OF CATALASE Aim : To study the effect of pH on the activity of catalase. Introduction : Catalase‚ an enzyme found in many different tissues‚ catalyses the breakdown of hydrogen peroxide into water and oxygen. 2 H202 → 2H20 + O2 Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product. The activity of catalase can be measured by finding the rate of oxygen release from hydrogen peroxide
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Core Practical: Enzyme concentrations and enzyme activity. Introduction In this experiment I shall investigate how the enzyme concentration can affect the initial rate of reaction. I will measure the effect of the enzyme in 5 different concentrations against the controlled variable of the reactant. The enzyme which will be used is different concentrations of potato and the reactant used will be Hydrogen Peroxide. Hydrogen Peroxide which will be the buffer solution is a PH of 7.2. My hypothesis
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