Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this
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Introductory Biology 1 Biology 1003 Fall Term 2011 Lab Number: 3 Title: Cell Energetics: Enzyme Role in Biological Reactions Name: Brandon Moore Student Number: 100819124 Lab day and time: Wednesday pm Date: Wednesday November 23‚ 2011 Introduction Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology
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Factor Affecting Enzyme Action | By: Jerry Li 02/03/2013 02/03/2013 | | HL Biology Term 1 Year 11 HL Biology H Contents 1 Design 2 1.1 Defining the problem 2 Research Question 2 Hypothesis 2 Background Information 2 Investigation Variables 3 1.2 Controlling Variables 4 Control Variables 4 Uncontrolled Variables 4 Control Experiment 4 1.3 Experimental
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Rate of Reaction that Enzyme Concentration‚ pH‚ and Temperatures Have on the Amylase Enzymes Color Disappearance Abstract: Compare reaction rates of the concentrations‚ pH’s‚ and temperatures of the enzyme Amylase. At what concentrates do the substrate molecules collide with each other‚ making the reaction possible? At what pH levels do the 3D molecular structures change breaking the H-bond and/or denaturize? At what temperatures do the collisions of the substrate molecules happen? Proteins
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Patrick McCrystal Enzymes: Natural Catalysts Enzymes are catalytic proteins‚ meaning they speed up chemical reactions without beingused up or altered permanently in the process. Although various enzymes use different methods‚all accomplish catalysis by lowering the activation energy for the reaction‚ thus allowing it tooccur more easily. Enzymes have very specific shapes (conformations). Part of the conformationis the active site of the enzyme‚ where the actual catalysis occurs. The specific molecule
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Enzyme Catalysis Lab Problem: Before the lab‚ one should understand: • The general functions and activities of enzymes; • The relationship between the structure and function of enzymes • The concept of initial reaction rates of enzymes; • How the concept of free energy relates to enzyme activity; • That change in temperature‚ pH‚ enzyme concentration‚ and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and • Catalyst‚ catalysis‚ and catalase
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Purpose: The purpose of this lab was to observe and understand the effects of changes in temperature‚ pH‚ enzyme concentration‚ and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Hypothesis: I believe that if there is an increase in enzyme concentration‚ an increase in temperature‚ or an increase in pH‚ then the intensity of the reaction will
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follows: “Does ethanol concentration affect the activity of bovine catalase?” It is not sufficient for the student merely to restate the research question provided by the teacher. Variables are factors that can be measured and/or controlled. Independent variables are those that are manipulated‚ and the result of this manipulation leads to the measurement of the dependent variable. A controlled variable is one that should be held constant so as not to obscure the effects of the independent variable
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The effect of time on enzyme reaction. Abstract: In this lab investigation we will observe how the amount of hydrogen peroxide is affected by catalase over time. The enzyme was added to 10 mL’s of hydrogen peroxide and observed over time to determine the relation between time and enzyme activity. The hypothesis stated that as time increased substrate would decrease. Therefore I predicted that at 60 seconds‚ there would be the least amount of H2O2. The enzyme activity mirrored my predictions
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Introduction: Enzymes are biological catalysts that permit cells to carry out the many functions that are required in a living system. Every enzyme has a specific substrate and a specific function. Enzymes alter substrates one of three ways: by adding something to the substrate‚ removing something from the substrate‚ or by changing its conformation‚ otherwise known as its shape. The structure of an enzyme and its ability to function exists because it binds to an active site‚ thus increasing the
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